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Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86

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Abstract

To obtain enzymatic preparations with higher laccase activity levels from Funalia floccosa LPSC 232, available for use in several applications, co-cultures with six filamentous microfungi were tested. A laccase non-producing soil fungus, identified as Penicillium commune GHAIE86, showed an outstanding ability to increase laccase activity (3-fold as compared to that for monoculture) when inoculated in 6-day-old F. floccosa cultures. Maximum laccase production with the F. floccosa and P. commune co-culture reached 60 U/mL, or twice that induced by chemical treatments alone. Our study demonstrated that co-culture with soil fungi might be a promising method for improving laccase production in F. floccosa. Although the enhancement of laccase activity was a function of P. commune inoculation time, two laccase isoenzymes produced by F. floccosa remained unchanged when strains were co-cultured. These data are compatible with the potential of F. floccosa in agricultural applications in soil, whose enzyme machinery could be activated by soil fungi such as P. commune.

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Acknowledgements

G. Heredia wishes to thank the Agencia Española de Cooperación Internacional (AECI-MAEC Program) for providing financial support for a sabbatical stay at the Department of Microbiology, Estación Experimental del Zaidín. M.C.N. Saparrat is a researcher from CONICET in Argentina. Inmaculada Sampedro wishes to thank MINECO for her “Ramón y Cajal” contract. The English text was corrected by Michael O’Shea.

Funding

This study was supported by Spanish projects AGL2008-00572/AGR and Prolipapel II S-2009AMB-1480 as well as the Agencia Nacional de Promoción Científíca y Tecnológica (PICT 2015-1620 to Saparrat, M.).

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Author notes

  1. Gabriela Heredia

    Present address: Institute of Ecology, A.C, Carretera antigua Xalapa-Coatepec 351, El Haya, 91070, Xalapa, Veracruz, Mexico

  2. José A. Siles

    Present address: Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020, Innsbruck, Austria

  3. Inmaculada Sampedro

    Present address: Biomedical Research Center (CIBM), Institute of Biotechnology, University of Granada, Granada, Spain

Authors and Affiliations

  1. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Spanish National Research Council (CSIC), Profesor Albareda 1, 18008, Granada, Spain

    Rosario Díaz Rodríguez, Gabriela Heredia, José A. Siles, Inmaculada García-Romera & Inmaculada Sampedro

  2. Department of Food Chemistry and Technology, ETSIAAB, Technical University of Madrid, Madrid, Spain

    Miguel Jurado

  3. Institute of Plant Physiology (INFIVE), National University of La Plata (UNLP)-CCT-La Plata-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Diag. 113 and 61, CC 327, 1900, La Plata, Argentina

    Mario Carlos Nazareno Saparrat

  4. Faculty of Pharmacy, Department of Microbiology, University of Granada, Granada, Spain

    Inmaculada Sampedro

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  1. Rosario Díaz Rodríguez
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  2. Gabriela Heredia
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  3. José A. Siles
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  4. Miguel Jurado
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  5. Mario Carlos Nazareno Saparrat
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  6. Inmaculada García-Romera
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  7. Inmaculada Sampedro
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Contributions

R.D. and G.H. contributed equally to this study.

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Correspondence to Inmaculada Sampedro.

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Rodríguez, R.D., Heredia, G., Siles, J.A. et al. Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86. Folia Microbiol 64, 91–99 (2019). https://doi.org/10.1007/s12223-018-0635-y

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