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Relation to enterocins of variable Aeromonas species isolated from trouts of Slovakian aquatic sources and detected by MALDI-TOF mass spectrometry

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Abstract

Aeromonads represent bacteria thought to be primarily mostly autochthonous to aquatic environments. This study was focused on the relation with antibiotics and enterocins of identified Aeromonas species isolated from the intestine of trouts living in Slovakian aquatic sources. Intestinal samples from 50 trouts (3 Salmo trutta and 47 Salmo gairdnerii) were collected in April of years 2007, 2010, and 2015 from trouts of different water sources in Slovakia (pond Bukovec near Košice, river Čierny Váh). Due to the MALDI-TOF mass spectrometry evaluation, 25 strains were proposed to the genus Aeromonas involving nine different species (Aeromonas bestiarum—nine strains, Aer. salmonicida—four strains, Aer. encheleia, Aer. eucrenophila, Aer. molluscorum, Aer. media, Aer. sobria, Aer. popoffii, Aer. veronii). Phenotypic evaluation of individual strains confirmed their species identification. Twenty-five strains of different Aeromonas species were sensitive to azithromycin, amikacin, mecillinam, mezlocillin, piperacillin, gentamicin, chloramphenicol, and tetracycline. On the other side, they were resistant to carbenicillin and ticarcillin. The growth of Aer. bestiarum R41/1 was inhibited by treatment with Ent M and Ent 2019 (inhibition activity 100 AU/mL). Aer. bestiarum R47/3 was inhibited by eight enterocins (100 AU/mL). It is the first study testing enterocins to inhibit the growth of Aeromonas species from trouts.

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Acknowledgements

This study was partially supported by the project VEGA 2/0006/17. We would like to thank for excellent laboratory work Mrs. Margita Bodnárová.

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Authors and Affiliations

  1. Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia

    Andrea Lauková, Ivana Kubašová, Anna Kandričáková, Viola Strompfová & Monika Pogány Simonová

  2. National Agricultural and Food Centre, Hlohovecká 2, 951 41 Nitra-Lužianky, Košice, Slovakia

    Rudolf Žitňan

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  1. Andrea Lauková
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  2. Ivana Kubašová
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  3. Anna Kandričáková
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  4. Viola Strompfová
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  5. Rudolf Žitňan
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  6. Monika Pogány Simonová
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Correspondence to Andrea Lauková.

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Lauková, A., Kubašová, I., Kandričáková, A. et al. Relation to enterocins of variable Aeromonas species isolated from trouts of Slovakian aquatic sources and detected by MALDI-TOF mass spectrometry. Folia Microbiol 63, 749–755 (2018). https://doi.org/10.1007/s12223-018-0616-1

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  • DOI: https://doi.org/10.1007/s12223-018-0616-1

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