Abstract
Melanoma is the least common form of skin tumor, but it is potentially the most dangerous and responsible for the majority of skin cancer deaths. We suggest that the skin microbiome might be changed during the progression of melanoma. The aim of this study is to compare the composition of the skin microbiota between different locations (skin and melanoma) of a MeLiM (Melanoma-bearing Libechov Minipig) pig model (exophytic melanoma). Ninety samples were used for PCR-DGGE analysis with primers specifically targeting the V3 region of the 16S rRNA gene. The profiles were used for cluster analysis by UPGMA and principal coordinate analysis PCoA and also to calculate the diversity index (Simpson index of diversity). By comparing the obtained results, we found that both bacterial composition and diversity were significantly different between the skin and melanoma microbiomes. The abundances of Fusobacterium and Trueperella genera were significantly increased in melanoma samples, suggesting a strong relationship between melanoma development and skin microbiome changes.
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Acknowledgements
This work was supported by grants CIGA 20162019 and 20162001 of the Grant Agency of Czech University of Life Sciences Prague, Project Excellence CZ.02.1.01/0.0/0.0/15_003/0000460 and also by the Ministry of Education, Youth and Sports of the Czech Republic (project number LO1609) under the NPU I program.
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Mrázek, J., Mekadim, C., Kučerová, P. et al. Melanoma-related changes in skin microbiome. Folia Microbiol 64, 435–442 (2019). https://doi.org/10.1007/s12223-018-00670-3
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DOI: https://doi.org/10.1007/s12223-018-00670-3