Clinical strains of Lactobacillus reduce the filamentation of Candida albicans and protect Galleria mellonella against experimental candidiasis

Rossoni, Rodnei Dennis; dos Santos Velloso, Marisol; Figueiredo, Lívia Mara Alves; Martins, Carolina Pistille; Jorge, Antonio Olavo Cardoso; Junqueira, Juliana Campos

doi:10.1007/s12223-017-0569-9

Original Article
Published: 23 November 2017

Clinical strains of Lactobacillus reduce the filamentation of Candida albicans and protect Galleria mellonella against experimental candidiasis

Rodnei Dennis Rossoni ORCID: orcid.org/0000-0002-9977-3040¹,
Marisol dos Santos Velloso¹,
Lívia Mara Alves Figueiredo¹,
Carolina Pistille Martins¹,
Antonio Olavo Cardoso Jorge¹ &
Juliana Campos Junqueira¹

Folia Microbiologica volume 63, pages 307–314 (2018)Cite this article

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Abstract

Candida albicans is the most common human fungal pathogen and can grow as yeast or filaments, depending on the environmental conditions. The filamentous form is of particular interest because it can play a direct role in adherence and pathogenicity. Therefore, the purpose of this study was to evaluate the effects of three clinical strains of Lactobacillus on C. albicans filamentation as well as their probiotic potential in pathogen-host interactions via an experimental candidiasis model study in Galleria mellonella. We used the reference strain Candida albicans ATCC 18804 and three clinical strains of Lactobacillus: L. rhamnosus strain 5.2, L. paracasei strain 20.3, and L. fermentum strain 20.4. First, the capacity of C. albicans to form hyphae was tested in vitro through association with the Lactobacillus strains. After that, we verified the ability of these strains to attenuate experimental candidiasis in a Galleria mellonella model through a survival curve assay. Regarding the filamentation assay, a significant reduction in hyphae formation of up to 57% was observed when C. albicans was incubated in the presence of the Lactobacillus strains, compared to a control group composed of only C. albicans. In addition, when the larvae were pretreated with Lactobacillus spp. prior to C. albicans infection, the survival rate of G. mellonela increased in all experimental groups. We concluded that Lactobacillus influences the growth and expression C. albicans virulence factors, which may interfere with the pathogenicity of these microorganisms.

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Funding

This study was supported by the São Paulo Council of Research—FAPESP, Brazil (Grants 2013/25181-8 and 2015/09770-9).

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Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, São Paulo, Brazil
Rodnei Dennis Rossoni, Marisol dos Santos Velloso, Lívia Mara Alves Figueiredo, Carolina Pistille Martins, Antonio Olavo Cardoso Jorge & Juliana Campos Junqueira

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Rodnei Dennis Rossoni
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Marisol dos Santos Velloso
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Lívia Mara Alves Figueiredo
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Carolina Pistille Martins
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Antonio Olavo Cardoso Jorge
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Juliana Campos Junqueira
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Correspondence to Rodnei Dennis Rossoni.

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This study has been approved by the Ethics Committee of São Paulo State University (Unesp) under protocol 560.479.

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The authors declare that they have no conflict of interest.

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Rossoni, R.D., dos Santos Velloso, M., Figueiredo, L.M. et al. Clinical strains of Lactobacillus reduce the filamentation of Candida albicans and protect Galleria mellonella against experimental candidiasis. Folia Microbiol 63, 307–314 (2018). https://doi.org/10.1007/s12223-017-0569-9

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Received: 31 July 2017
Accepted: 15 November 2017
Published: 23 November 2017
Issue Date: May 2018
DOI: https://doi.org/10.1007/s12223-017-0569-9