Analysis of Streptomyces coelicolor M145 genes SCO4164 and SCO5854 encoding putative rhodaneses
Streptomyces coelicolor genome carries two apparently paralogous genes, SCO4164 and SCO5854, that encode putative thiosulfate sulfurtransferases (rhodaneses). These genes (and their presumed translation products) are highly conserved and widely distributed across actinobacterial genomes. The SCO4164 knockout strain was unable to grow on minimal media with either sulfate or sulfite as the sole sulfur source. The SCO5854 mutant had no growth defects in the presence of various sulfur sources; however, it produced significantly less amounts of actinorhodin. Furthermore, we discuss possible links between basic interconversions of inorganic sulfur species and secondary metabolism in S. coelicolor.
This work was supported by grants Bg-46F (to V.O.) and Bg-41Nr (to B.O.) from Ministry of Education and Science of Ukraine. T.G. was supported by DAAD fellowships. Prof. Paul Dyson (Swansea University, UK) is thanked for cosmids from S. coelicolor transposon library. We thank Lijiang Song (Univerity of Warwick, UK) for proofreading the manuscript.
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