Folia Microbiologica

, Volume 61, Issue 5, pp 431–438 | Cite as

Lipid accumulation by oleaginous and non-oleaginous yeast strains in nitrogen and phosphate limitation

  • Irena Kolouchová
  • Olga Maťátková
  • Karel Sigler
  • Jan Masák
  • Tomáš Řezanka


We investigated the possibility of utilizing both oleaginous yeast species accumulating large amounts of lipids (Yarrowia lipolytica, Rhodotorula glutinis, Trichosporon cutaneum, Candida sp.) and traditional biotechnological non-oleaginous ones characterized by high biomass yield (Kluyveromyces polysporus, Torulaspora delbrueckii, Saccharomyces cerevisiae) as potential producers of biofuel-utilizable and nutritionally valuable lipids. The main objective was to increase lipid accumulation by increasing C/P ratio together with higher C/N ratio, while maintaining high biomass yield. The C/N ratio of 30 was found to lead to higher biomass content and the total lipid content increased significantly with higher C/P ratio. With higher ratios of both C/N and C/P, the content of monounsaturated fatty acids (FAs) in cell lipids increased while polyunsaturated FAs decreased. Oleaginous yeast species had a lower proportion of unsaturated FAs (approx. 80 %) than non-oleaginous strains (approx. 90 %). At a C/N ratio of 30 and C/P ratio 1043, T. cutaneum produced a high amount of ω-6 unsaturated linoleic acid, the precursor of some prostaglandins, leukotrienes, and thromboxanes, while Candida sp. and K. polysporus accumulated a high content of palmitoleic acid.


Lipid Accumulation Total Lipid Content Palmitoleic Acid Lipid Yield Oleaginous Yeast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The research was supported by Czech Science Foundation (GACR) project P503 14-00227S.


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2016

Authors and Affiliations

  1. 1.Department of BiotechnologyUniversity of Chemistry and TechnologyPrague 6Czech Republic
  2. 2.Institute of MicrobiologyCASPragueCzech Republic

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