Decontamination by Persteril 36 may affect the reliability of DNA-based detection of biological warfare agents—short communication

Abstract

Persteril 36 is a disinfectant with a broad spectrum of antimicrobial activity. Because of its bactericidal, virucidal, fungicidal, and sporicidal effectiveness, it is used as a disinfectant against biological warfare agents in the emergency and army services. In case of an attack with potentially harmful biological agents, a person’s gear or afflicted skin is sprayed with a diluted solution of Persteril 36 as a precaution. Subsequently, the remains of the biological agents are analyzed. However, the question remains concerning whether DNA can be successfully analyzed from Persteril 36-treated dead bacterial cells. Spore-forming Bacillus subtilis and Gram-negative Pseudomonas aeruginosa and Xanthomonas campestris were splattered on a camouflage suit and treated with 2 or 0.2 % Persteril 36. After the disinfectant vaporized, the bacterial DNA was extracted and quantified by real-time PCR. A sufficient amount of DNA was recovered for downstream analysis only in the case of spore-forming B. subtilis treated with a 0.2 % solution of Persteril 36. The bacterial DNA was almost completely destroyed in Gram-negative bacteria or after treatment with the more concentrated solution in B. subtilis. This phenomenon can lead to false-negative results during the identification of harmful microorganisms.

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References

  1. Alasri A, Roques C, Michel G, Cabassud C, Aptel P (1992) Bactericidal properties of peracetic acid and hydrogen peroxide, alone and in combination, and chlorine and formaldehyde against bacterial water strains. Can J Microbiol 38:635–642

    CAS  Article  PubMed  Google Scholar 

  2. Baldry MGC (1983) The bactericidal, fungicidal and sporicidal properties of hydrogen peroxide and peracetic acid. J Appl Bacteriol 54:417–423

    CAS  Article  PubMed  Google Scholar 

  3. Buhr T, Wells C, Young A, Minter Z, Johnson C, Payne A, McPherson D (2013) Decontamination of materials contaminated with Bacillus anthracis and Bacillus thuringiensis Al Hakam spores using PES-Solid, a solid source of peracetic acid. J Appl Microbiol 115:398–408

    CAS  Article  PubMed  Google Scholar 

  4. CZ M (2006) Catalogue file of the typified activity of the integrated rescue system IZS STČ 05/IZS : finding of the object suspected to presence of B-agens or toxins. Prague

  5. Fernández A, Álvarez-Ordóñez A, López M, Bernardo A (2009) Effects of organic acids on thermal inactivation of acid and cold stressed Enterococcus faecium. Food Microbiol 26:497–503. doi:10.1016/j.fm.2009.03.002

    Article  PubMed  Google Scholar 

  6. Flores MJ, Lescano MR, Brandi RJ, Cassano AE, Labas MD (2014) A novel approach to explain the inactivation mechanism of Escherichia coli employing a commercially available peracetic acid. Water Sci Technol 69:358–363. doi:10.2166/wst.2013.721

    CAS  Article  PubMed  Google Scholar 

  7. Kitis M (2004) Disinfection of wastewater with peracetic acid: a review. Environ Int 30:47–55. doi:10.1016/S0160-4120(03)00147-8

    CAS  Article  PubMed  Google Scholar 

  8. Kouba A, Kuklina I, Niksirat H, Máchová J, Kozák P (2012) Tolerance of signal crayfish (Pacifastacus leniusculus) to Persteril 36 supports use of peracetic acid in astaciculture. Aquaculture 350–353:71–74. doi:10.1016/j.aquaculture.2012.04.016

    Article  Google Scholar 

  9. Ludík T, Barta J (2011) Architecture for operational processes improvement in emergency management. Recent researches in computational intelligence and information security. WSEAS Press.

  10. Ludík T, Barta J, Navrátil J (2013) Design patterns for emergency management processes. In: World Academy of science, engineering and technology. Int J Soc, Behav, Educ, Econ, Bus Ind Eng 7:1749–1756

    Google Scholar 

  11. Madsen AM, Zervas A, Tendal K, Nielsen JL (2015) Microbial diversity in bioaerosol samples causing ODTS compared to reference bioaerosol samples as measured using Illumina sequencing and MALDI-TOF. Environ Res 140:255–267. doi:10.1016/j.envres.2015.03.027

    CAS  Article  PubMed  Google Scholar 

  12. March JK et al (2015) The differential effects of heat-shocking on the viability of spores from Bacillus anthracis, Bacillus subtilis, and Clostridium sporogenes after treatment with peracetic acid- and glutaraldehyde-based disinfectants. Microbiol Open 4:764–773. doi:10.1002/mbo3.277

    CAS  Article  Google Scholar 

  13. Melichercikova V (1988) Disinfectant effect of Persteril in combination with detergents. J Hyg Epid Microb Im 33:19–28

    Google Scholar 

  14. Park E, Lee C, Bisesi M, Lee J (2014) Efficiency of peracetic acid in inactivating bacteria, viruses, and spores in water determined with ATP bioluminescence, quantitative PCR, and culture-based methods. J Water Health 12:13–23. doi:10.2166/wh.2013.002

    CAS  Article  PubMed  Google Scholar 

  15. Pazienza M et al (2014) Use of particle counter system for the optimization of sampling, identification and decontamination procedures for biological aerosols dispersion in confined environment. J Microbial Biotech 6:043–048. doi:10.4172/1948-5948.1000120

    Google Scholar 

  16. Rokhina EV, Makarova K, Golovina EA, Van As H, Virkutyte J (2010) Free radical reaction pathway, thermochemistry of peracetic acid homolysis, and its application for phenol degradation: spectroscopic study and quantum chemistry calculations. Environ Sci Technol 44:6815–6821. doi:10.1021/es1009136

    CAS  Article  PubMed  Google Scholar 

  17. Setlow P (2006) Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals. J Appl Microbiol 101:514–525. doi:10.1111/j.1365-2672.2005.02736.x

    CAS  Article  PubMed  Google Scholar 

  18. Sherry ST, Ward M-H, Kholodov M, Baker J, Phan L, Smigielski EM, Sirotkin K (2001) dbSNP: the NCBI database of genetic variation. Nucleic Acids Res 29:308–311

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  19. Talbot SR, Russmann H, Köhne S, Niederwöhrmeier B, Grote G, Scheper T (2010) Effects of inactivation methods on the analysis of Bacillus atrophaeus endospores using real-time PCR and MALDI-TOF-MS. Eng Life Sci 10:109–120. doi:10.1002/elsc.200800078

    CAS  Google Scholar 

  20. The Royal Society (2004) Making the UK safer: detecting and decontaminating chemical and biological agents. The Royal Society, London

    Google Scholar 

  21. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25(24):4876–82

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  22. Tran-Hung L, Tran-Thi N, Aboudharam G, Raoult D, Drancourt M (2007) A new method to extract dental pulp DNA: application to universal detection of bacteria. PLoS ONE 2:e1062. doi:10.1371/journal.pone.0001062

    Article  PubMed  PubMed Central  Google Scholar 

  23. UK Government Decontamination Service (2015) Strategic national guidance: the decontamination of buildings, infrastructure and open environment exposed to chemical, biological, radiological substances or nuclear (CBRN) materials. UK Government Decontamination Service, London

    Google Scholar 

  24. Wood J, Calfee M, Clayton M, Griffin-Gatchalian N, Touati A, Egler K (2013) Evaluation of peracetic acid fog for the inactivation of Bacillus anthracis spore surrogates in a large decontamination chamber. J Hazard Mater 250–251:61–67

    Article  PubMed  Google Scholar 

  25. Zhao X, Lin CW, Wang J, Oh DH (2014) Advances in rapid detection methods for foodborne pathogens. J Microbiol Biotechn 24:297–312. doi:10.4014/jmb.1310.10013

    CAS  Article  Google Scholar 

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Acknowledgments

This project was supported by the Czech Science Foundation, grant no. 14-36938G, and by the Ministry of the Interior of the Czech Republic, grant VF20122015024.

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Correspondence to Daniel Vanek.

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Josefiova, J., Pospisek, M. & Vanek, D. Decontamination by Persteril 36 may affect the reliability of DNA-based detection of biological warfare agents—short communication. Folia Microbiol 61, 417–421 (2016). https://doi.org/10.1007/s12223-016-0451-1

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Keywords

  • Peracetic Acid
  • Chlorine Dioxide
  • Decontamination Procedure
  • Neutralization Step
  • Biological Warfare Agent