Enhancing the lipid productivity of yeasts with trace concentrations of iron nanoparticles
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Oxidative stress induced by zero-valent iron nanoparticles (nZVIs) was used to improve lipid accumulation in various oleaginous and non-oleginous yeasts—Candida sp., Kluyveromyces polysporus, Rhodotorula glutinis, Saccharomyces cerevisiae, Torulospora delbrueckii, Trichosporon cutaneum, and Yarrowia lipolytica. The highest lipid yields occurred at 9–13 mg/L nZVIs. Gas chromatography-mass spectrometry was used for the quantitative and qualitative analysis of the fatty acids. It showed an increasing abundance of polyunsaturated fatty acids, especially essential linoleic acid, in the presence of nZVIs. Our results suggest that nZVIs can be used to improve not only lipid production by oleaginous microorganisms but also the nutritional value of biosynthesized unsaturated fatty acids.
KeywordsFatty Acid Composition Lipid Accumulation Yeast Species Lipid Production Oleaginous Yeast
The research was supported by GACR 14-00227S, by Financial support from specific university research (MSMT No 20/2015), and by Competence Center TE01020218 of the Technology Agency of the Czech Republic.
- Dembitsky VM, Řezanka T (2005) Metabolites produced by nitrogen-fixing Nostoc species. Folia Microbiol 50:363–391Google Scholar
- Philpott CC (2006) Iron uptake in fungi: a system for every source. BBA Mol Cell Res 1763:636–645Google Scholar
- Šmilauer P, Lepš J (2003) Multivariate analysis of ecological data using CANOCO 5. Cambridge university press, CambridgeGoogle Scholar
- Yilancioglu K, Cokol M, Pastirmaci I, Erman B, Cetiner S (2014) Oxidative stress is a mediator for increased lipid accumulation in a newly isolated Dunaliella salina strain. Plos One 9(e91957):1–13Google Scholar