Folia Microbiologica

, Volume 61, Issue 3, pp 221–226 | Cite as

Molecular characterization of bacteremic Escherichia coli isolates in Romania

  • Codruţa-Romaniţa UseinEmail author
  • Raluca Papagheorghe
  • Mihaela Oprea
  • Maria Condei
  • Monica Strãuţ


The increasing prevalence of invasive infections caused by antibiotic resistant Escherichia coli strains in Romanian patients, already mentioned in the European reports, requires better knowledge of their specific traits. Thus, a set of 38 E. coli blood isolates, collected between 2010 and 2012 at one of the local hospitals participating into the European Antimicrobial Resistance Surveillance Network, was investigated retrospectively with respect to the phylogenetic origin, extraintestinal virulence-associated markers (i.e. fimH, papC, papG alleles, sfa/foc, afa/dra, hly, cnf1, sat, iucC, fyuA, ibeA), and beta-lactamase encoding genes (i.e. bla CTX-M, bla TEM, and bla SHV alleles). The isolates with extended-spectrum beta-lactamase (ESBL) phenotypes were further characterized using PCR-based replicon typing and multilocus sequencing typing. For ST131 members, pulsed-field gel electrophoresis (PFGE) and PCR-based detection of fimH30 allele were performed. Overall, the isolates were more likely members of the major phylogenetic group A (53 %) and to a lesser extent of groups B2 (29 %), D (10 %), and B1 (8 %). All but three of the virulence markers sought (i.e. papGI, hly, cnf1) were detected with prevalence ranging from 3 % (i.e. ibeA, papGIII) to 87 % (fimH). As expected, the most complex genotypes (four to seven virulence markers) defined the isolates derived from phylogenetic groups B2 and D. ESBL producers were bla CTX-M-15-positive, mostly of phylogroup A (67 %), harboured IncF multireplicon plasmids, and belonged to six sequence types (i.e. ST10, ST131, ST167, ST410, ST540, ST1275). Members of ST10 clonal complex (i.e. ST10, ST167) were the most common. The ST131 isolates belonged to H30 subclone and displayed 74 % similarity at PFGE analysis.


Nitrofuran Multilocus Sequence Typing Virulence Marker Dice Similarity Coefficient ST131 Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This material is based upon the work supported by the Ministry of Education and Scientific Research through NUCLEU Programme, project PN 09-22 01 01.


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2015

Authors and Affiliations

  • Codruţa-Romaniţa Usein
    • 1
    • 2
    Email author
  • Raluca Papagheorghe
    • 3
  • Mihaela Oprea
    • 1
  • Maria Condei
    • 1
  • Monica Strãuţ
    • 1
  1. 1.Cantacuzino National Institute of Research-Development for Microbiology and ImmunologyBucharestRomania
  2. 2.Carol Davila University of Medicine and PharmacyBucharestRomania
  3. 3.Colţea Clinical HospitalBucharestRomania

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