Advertisement

Folia Microbiologica

, Volume 61, Issue 3, pp 221–226 | Cite as

Molecular characterization of bacteremic Escherichia coli isolates in Romania

  • Codruţa-Romaniţa UseinEmail author
  • Raluca Papagheorghe
  • Mihaela Oprea
  • Maria Condei
  • Monica Strãuţ
Article

Abstract

The increasing prevalence of invasive infections caused by antibiotic resistant Escherichia coli strains in Romanian patients, already mentioned in the European reports, requires better knowledge of their specific traits. Thus, a set of 38 E. coli blood isolates, collected between 2010 and 2012 at one of the local hospitals participating into the European Antimicrobial Resistance Surveillance Network, was investigated retrospectively with respect to the phylogenetic origin, extraintestinal virulence-associated markers (i.e. fimH, papC, papG alleles, sfa/foc, afa/dra, hly, cnf1, sat, iucC, fyuA, ibeA), and beta-lactamase encoding genes (i.e. bla CTX-M, bla TEM, and bla SHV alleles). The isolates with extended-spectrum beta-lactamase (ESBL) phenotypes were further characterized using PCR-based replicon typing and multilocus sequencing typing. For ST131 members, pulsed-field gel electrophoresis (PFGE) and PCR-based detection of fimH30 allele were performed. Overall, the isolates were more likely members of the major phylogenetic group A (53 %) and to a lesser extent of groups B2 (29 %), D (10 %), and B1 (8 %). All but three of the virulence markers sought (i.e. papGI, hly, cnf1) were detected with prevalence ranging from 3 % (i.e. ibeA, papGIII) to 87 % (fimH). As expected, the most complex genotypes (four to seven virulence markers) defined the isolates derived from phylogenetic groups B2 and D. ESBL producers were bla CTX-M-15-positive, mostly of phylogroup A (67 %), harboured IncF multireplicon plasmids, and belonged to six sequence types (i.e. ST10, ST131, ST167, ST410, ST540, ST1275). Members of ST10 clonal complex (i.e. ST10, ST167) were the most common. The ST131 isolates belonged to H30 subclone and displayed 74 % similarity at PFGE analysis.

Keywords

Nitrofuran Multilocus Sequence Typing Virulence Marker Dice Similarity Coefficient ST131 Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This material is based upon the work supported by the Ministry of Education and Scientific Research through NUCLEU Programme, project PN 09-22 01 01.

References

  1. Banerjee R, Johnston B, Lohse C, Porter SB, Clabots C, Johnson JR (2013) Escherichia coli sequence type 131 is a dominant, antimicrobial-resistant clonal group associated with healthcare and elderly hosts. Infect Control Hosp Epidemiol 34(4):361–369. doi: 10.1086/669865 CrossRefPubMedPubMedCentralGoogle Scholar
  2. Bergeron CR, Prussing C, Boerlin P, Daignault D, Dutil L, Reid-Smith RJ, Zhanel GG, Manges AR (2012) Chicken as reservoir for extraintestinal pathogenic Escherichia coli in humans, Canada. Emerg Infect Dis 18(3):415–421. doi: 10.3201/eid1803.111099 CrossRefPubMedPubMedCentralGoogle Scholar
  3. Bert F, Huynh B, Dondero F, Johnson JR, Paugam-Burtz C, Durand F, Belghiti J, Valla D, Moreau R, Nicolas-Chanoine MH (2011) Molecular epidemiology of Escherichia coli bacteremia in liver transplant recipients. Transpl Infect Dis 13(4):359–365. doi: 10.1111/j.1399-3062.2011.00618.x CrossRefPubMedGoogle Scholar
  4. Carattoli A (2009) Resistance plasmid families in Enterobacteriaceae. Antimicrob Agents Chemother 53(6):2227–38. doi: 10.1128/AAC.01707-0814 CrossRefPubMedPubMedCentralGoogle Scholar
  5. Clermont O, Bonacorsi S, Bingen E (2000) Rapid and simple detection of the Escherichia coli phylogenetic group. Appl Environ Microbiol 66(10):4555–4558. doi: 10.1128/AEM.66.10.4555-4558.2000 CrossRefPubMedPubMedCentralGoogle Scholar
  6. Colpan A, Johnston B, Porter S, Clabots C, Anway R, Thao L, Kuskowski MA, Tchesnokova V, Sokurenko EV, Johnson JR, and on behalf of the VICTORY (Veterans Influence of Clonal Types on Resistance: Year 2011) Investigators (2013) Escherichia coli sequence type 131 (ST131) subclone H30 as an emergent multidrug-resistant pathogen among US veterans. Clin Infect Dis 57(9):1256–1265. doi: 10.1093/cid/cit503
  7. Cooke NM, Smith SG, Kelleher M, Rogers TR (2010) Major differences exist in frequencies of virulence factors and multidrug resistance between community and nosocomial Escherichia coli bloodstream isolates. J Clin Microbiol 48(4):1099–104. doi: 10.1128/JCM.02017-09 CrossRefPubMedPubMedCentralGoogle Scholar
  8. Johnson JR, Russo TA (2002) Extraintestinal pathogenic Escherichia coli: “the other bad E coli”. J Lab Clin Med 139(3):155–162. doi: 10.1067/mlc.2002.121550 CrossRefPubMedGoogle Scholar
  9. Johnson J, Menard M, Johnston B, Kuskowski M, Nichol K, Zhanel G (2009) Epidemic clonal groups of Escherichia coli as a cause of antimicrobial-resistant urinary tract infections in Canada, 2002–2004. Antimicrob Agents Chemother 53(7):2733–2739. doi: 10.1128/AAC.00297-09 CrossRefPubMedPubMedCentralGoogle Scholar
  10. Markovska R, Schneider I, Keuleyan E, Sredkova M, Ivanova D, Markova B, Lazarova G, Dragijeva E, Savov E, Haydouchka I, Hadjieva N, Setchanova L, Mitov I, Bauernfeind A (2008) Extended-spectrum beta-lactamase-producing Enterobacteriaceae in Bulgarian hospitals. Microb Drug Resist 14(2):119–128. doi: 10.1089/mdr.2008.0814 CrossRefPubMedGoogle Scholar
  11. Mokady D, Gophna U, Ron EZ (2005) Virulence factors of septicemic Escherichia coli strains. Int J Med Microbiol 295(6–7):455–62. doi: 10.1016/j.ijmm.2005.07.007 CrossRefPubMedGoogle Scholar
  12. Nicolas-Chanoine MH, Blanco J, Leflon-Guibout V, Demarty R, Alonso MP, Caniça MM, Park YJ, Lavigne JP, Pitout J, Johnson JR (2008) Intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15. J Antimicrob Chemother 61(2):273–281. doi: 10.1093/jac/dkm464 CrossRefPubMedGoogle Scholar
  13. Olesen B, Scheutz F, Menard M, Skov MN, Kolmos HJ, Kuskowski MA, Johnson JR (2009) Three-decade epidemiological analysis of Escherichia coli O15:K52:H1. J Clin Microbiol 47(6):1857–1862. doi: 10.1128/JCM.00230-09 CrossRefPubMedPubMedCentralGoogle Scholar
  14. Oteo J, Diestra K, Juan C, Bautista V, Novais A, Pérez-Vázquez M, Moyá B, Miró E, Coque TM, Oliver A, Cantón R, Navarro F, Campos J; Spanish Network in Infectious Pathology Project (REIPI) (2009) Extended-spectrum beta-lactamase-producing Escherichia coli in Spain belong to a large variety of multilocus sequence typing types, including ST10 complex/A, ST23 complex/A and ST131/B2. Int J Antimicrob Agents 34(2):173–176. doi: 10.1016/j.ijantimicag.2009.03.006 CrossRefGoogle Scholar
  15. Peirano G, van der Bij AK, Freeman JL, Poirel L, Nordmann P, Costello M, Tchesnokova VL, Pitout JD (2014) Characteristics of Escherichia coli sequence type 131 isolates that produce extended-spectrum β-lactamases: global distribution of the H30-Rx sublineage. Antimicrob Agents Chemother 58(7):3762–3767. doi: 10.1128/AAC.02428-14 CrossRefPubMedPubMedCentralGoogle Scholar
  16. Price LB, Johnson JR, Aziz M, Clabots C, Johnston B, Tchesnokova V, Nordstrom L, Billig M, Chattopadhyay S, Stegger M, Andersen PS, Pearson T, Riddell K, Rogers P, Scholes D, Kahl B, Keim P, Sokurenko EV (2013) The epidemic of extended-spectrum-lactamase-producing Escherichia coli ST131 is driven by a single highly pathogenic subclone, H30-Rx. mBio 4(6):e00377-13. doi: 10.1128/mBio.00377-13
  17. Schink AK, Kadlec K, Kaspar H, Mankertz J, Schwarz S (2013) Analysis of extended-spectrum-β-lactamase-producing Escherichia coli isolates collected in the GERM-Vet monitoring programme. J Antimicrob Chemother 68(8):1741–1749. doi: 10.1093/jac/dkt123 CrossRefPubMedGoogle Scholar
  18. Schmidt H, Hensel M (2004) Pathogenicity islands in bacterial pathogenesis. Clin Microbiol Rev 17(1):14–56. doi: 10.1128/CMR.17.1.14-56.2004 CrossRefPubMedPubMedCentralGoogle Scholar
  19. Székely E, Damjanova I, Jánvári L, Vas KE, Molnár S, Bilca DV et al (2013) First description of bla(NDM-1), bla(OXA-48), bla(OXA-181) producing Enterobacteriaceae strains in Romania. Int J Med Microbiol 303(8):697–700. doi: 10.1016/j.ijmm.2013.10.001 CrossRefPubMedGoogle Scholar
  20. Usein CR, Andrea L, Margareta R, Mãdãlina CM, Delia M (2011) Phylogenetic background and extraintestinal virulence genotypes of Escherichia coli vaginal strains isolated from adult women. Rev Romana Med Lab 19(1):37–45Google Scholar
  21. Usein CR, Condei M, Cristea D, Ciontea SA, Tatu-Chiţoiu D, Cristea V et al (2014) Escherichia coli ST131 causing invasive infections in Romanian patients—a threat we can no longer ignore. Roum Arch Microbiol Immunol 73(1–2):5–8PubMedGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2015

Authors and Affiliations

  • Codruţa-Romaniţa Usein
    • 1
    • 2
    Email author
  • Raluca Papagheorghe
    • 3
  • Mihaela Oprea
    • 1
  • Maria Condei
    • 1
  • Monica Strãuţ
    • 1
  1. 1.Cantacuzino National Institute of Research-Development for Microbiology and ImmunologyBucharestRomania
  2. 2.Carol Davila University of Medicine and PharmacyBucharestRomania
  3. 3.Colţea Clinical HospitalBucharestRomania

Personalised recommendations