Abstract
An arsenate susceptibility test was performed with transformed and cultured Escherichia coli DH5α cells, which carried recombinant DNA of full-length arsenic (ars) operon, namely a putative membrane permease, ArsP; a transcriptional repressor, ArsR; an arsenate reductase, ArsC; and an arsenical-resistance membrane transporter, Acr3, from the Japanese urease-positive thermophilic Campylobacter lari (UPTC) CF89-12. The E. coli DH5α transformant showed reduced susceptibility to arsenate (~1536 μg/mL), compared to the control. Thus, these ars four-genes from the UPTC CF89-12 strain cells could confer a reduced susceptibility to arsenate in the transformed and E. coli DH5α cells. E. coli transformants with truncated ars operons, acr3 (acr3) and arsC-acr3 (∆arsC-acr3), of the ars operon, showed an MIC value of 384 μg/mL (~384 μg/mL), similar to the E. coli cells which carried the pGEM-T vector (control). Reverse transcription PCR confirmed in vivo transcription of recombinant full-length ars operon and deletion variants (∆acr3 and ∆arsC-acr3) in the transformed E. coli cells.
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Acknowledgments
This research was partially supported by a Grant-in-Aid for Scientific Research (C) (no. 20580346) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to MM). MM and JEM were funded through a Great Britain Sasakawa Foundation (Butterfield) Award to jointly examine the clinical significance of Campylobacter infection in the UK and Japan.
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Matsuda, M., Kuribayashi, T., Yamamoto, S. et al. Transformation and characterization of an arsenic gene operon from urease-positive thermophilic Campylobacter (UPTC) in Escherichia coli . Folia Microbiol 61, 57–62 (2016). https://doi.org/10.1007/s12223-015-0405-z
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DOI: https://doi.org/10.1007/s12223-015-0405-z