Folia Microbiologica

, Volume 60, Issue 1, pp 1–5 | Cite as

Biofilm formation by Staphylococcus hominis strains isolated from human clinical specimens

  • Ewa Szczuka
  • Kinga Telega
  • Adam Kaznowski


Staphylococcus hominis is the third species of coagulase-negative staphylococci (CoNS) most frequently isolated from specimens of patients with hospital-acquired infections. Many infections caused by CoNS appeared to be associated with biofilms. Nevertheless, the knowledge of the ability of S. hominis to form a biofilm is limited. The aim of this study was to analyze the formation of the biofilm by 56 S. hominis strains isolated from clinical cases. The biofilm three-dimensional structure was reconstructed by confocal laser scanning microscopy. We found that most of S. hominis strains carried icaADBC genes encoding polysaccharide intercellular adhesin (PIA), which plays a crucial role in the formation of biofilms in staphylococci strains. However, only a half of the ica-positive strains had an ability to form a biofilm in vitro. In this study, we also accessed the sensitivity of biofilms of S. hominis strains to sodium metaperiodate, proteinase K and DNase. We found that polysaccharides and proteins are the major components of the extracellular matrix of the biofilm formed by S. hominis. DNase did not have a significant effect on biofilms, which suggested that nucleic acid plays a minor role in the mature biofilm.


Prosthetic Joint Infection NaIO4 Crystal Violet Solution Sodium Metaperiodate Polysaccharide Intercellular Adhesin 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2014

Authors and Affiliations

  1. 1.Department of Microbiology, Institute of Experimental Biology, Faculty of BiologyAdam Mickiewicz UniversityPoznańPoland

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