Abstract
A group of 29 isolates of Candida parapsilosis sensu stricto, 29 of Candida orthopsilosis, and 4 of Candida metapsilosis were assayed for the presence of killer activity using Saccharomyces cerevisiae ATCC 26609 as a sensitive strain. All C. metapsilosis isolates showed killer activity at 25 °C while strains of C. parapsilosis sensu stricto or C. orthopsilosis did not exhibit this activity. Sensitivity to killer toxins was evaluated using a set of previously reported killer strains of clinical origin. Only 11 isolates of the C. parapsilosis complex were inhibited by at least one killer isolate without resulting in any clear pattern, except for C. parapsilosis sensu stricto ATCC 22019, which was inhibited by every killer strain with the exception of C. parapsilosis and Candida utilis. The lack of sensitivity to killer activity among isolates of the genus Candida suggests that their toxins belong to the same killer type. Differentiation of species within the C. parapsilosis complex using the killer system may be feasible if a more taxonomically diverse panel of killer strains is employed.
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Arroyo-Helguera O, De Las Penas A, Castaño I (2012) Occurrence of killer Candida glabrata clinical isolates. Braz J Microbiol 43:880–887
Baeza ME, Sanhueza MA, Cifuentes VH (2008) Occurrence of killer yeast strains in industrial and clinical isolates. Biol Res 41:173–182
Boekhout T, Scorzetti G (1997) Differential killer toxin sensitivity patterns of varieties of Cryptococcus neoformans. J Med Vet Mycol 35:147–149
Buzzini P, Martini A (2000) Differential growth inhibition as a tool to increase the discriminating power of killer toxin sensitivity in fingerprinting of yeasts. FEMS Microbiol Lett 193:31–36
Buzzini P, Martini A (2001) Discrimination between Candida albicans and other pathogenic species of the genus Candida by their differential sensitivities to toxins of a panel of killer yeasts. J Clin Microbiol 39:3362–3364
Buzzini P, Berardinelli S, Turchetti B et al (2003) Fingerprinting of yeasts at the strain level by differential sensitivity responses to a panel of selected killer toxins. Syst Appl Microbiol 26:466–470
Buzzini P, Turchetti B, Vaughan-Martini AE (2007) The use of killer sensitivity patterns for biotyping yeast strains: the state of the art, potentialities and limitations. FEMS Yeast Res 7:749–760
Cantón E, Pemán J, Quindón G et al (2011) Prospective multicenter study of the epidemiology, molecular identification, and antifungal susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis isolated from patients with candidemia. Antimicrob Agents Chemother 55:5590–5596
Conti S, Cantelli C, Gerloni M et al (1996) Killer factor interference in mixed opportunistic yeast cultures. Mycopathologia 135:1–8
Coutinho SD, Paula CR (1998) Biotyping of Malassezia pachydermatis strains using the killer system. Rev Iberoam Micol 15:85–87
Golubev WI (1998) In: Kurtzman CP, Fell JW (eds) Mycocins (killer toxins). The yeasts. A taxonomic study. Academic Press, London, pp 55–62
Kandel JS, Stern TA (1997) Killer phenomenon in pathogenic yeast. Antimicrob Agents Chemother 15:568–571
Morace G, Manzara S, Dettori G et al (1989) Biotyping of bacterial isolates using the yeast killer system. Eur J Epidemiol 5:303–310
Ozhovan IM, Arzumanian VG, Basnak’ian IA (2002) Killer toxins of clinically important yeasts. Zh Mikrobiol Epidemiol Immunobiol 5:79–83
Polonelli L, Morace G (1987) Production and characterization of yeast killer toxin monoclonal antibodies. J Clin Microbiol 25:460–462
Polonelli L, Morace G (1988) Yeast killer toxin-like anti-idiotypic antibodies. J Clin Microbiol 26:602–604
Polonelli L, Dettori G, Cattel C, Morace G (1987) Biotyping of micelial fungus cultures by the killer system. Eur J Epidemiol 3:237–242
Polonelli L, Magliani W, Conti S et al (2003) Therapeutic activity of an engineered synthetic killer antiidiotypic antibody fragment against experimental mucosal and systemic candidiasis. Infect Immun 71:6205–6212
Robledo-Leal E, Villarreal-Treviño L, González GM (2012) Occurrence of killer yeasts in isolates of clinical origin. Trop Biomed 29:297–300
Santos A, Sánchez A, Marquina D (2004) Yeasts as biological agents to control Botrytis cinerea. Microbiol Res 159:331–338
Scheid LA, Mario DAN, Heins-Vaccari EM et al (2010) Differentiation of Candida dubliniensis from Candida albicans with the use of killer toxins. Rev Inst Med Trop Sao Paulo 52:161–162
Treviño-Rangel RDJ, Garza-González E, González JG et al (2012) Molecular characterization and antifungal susceptibility of the Candida parapsilosis species complex of clinical isolates from Monterrey, Mexico. Med Mycol 50:781–784
Zekhnov AM, Soom YO, Nesterova GF (1989) New test strains for detecting the antagonistic activity of yeasts. Mikrobiologiya 58:807–811
Acknowledgments
This study was supported by the PAICYT program of our university (Project No. CN986-11). We would like to thank Sergio Lozano, M.D., for his review of the manuscript in English.
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Robledo-Leal, E., Elizondo-Zertuche, M., Villarreal-Treviño, L. et al. Killer behavior within the Candida parapsilosis complex. Folia Microbiol 59, 503–506 (2014). https://doi.org/10.1007/s12223-014-0327-1
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DOI: https://doi.org/10.1007/s12223-014-0327-1