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Antimicrobial activity of tigecycline alone or in combination with rifampin against Staphylococcus epidermidis in biofilm

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Abstract

Staphylococcus epidermidis is a commensal inhabitant of the healthy human skin, but in the recent years, it has been recognized as a nosocomial pathogen especially in immunocompromised patients. The pathogenesis of S. epidermidis is thought to be based on its capacity to form biofilms on the surface of medical devices, where bacterial cells may persist, protected from host defence and antimicrobial agents. Rifampin has been shown to be one of the most active antimicrobial agents in the eradication of the staphylococcal biofilm. However, this antibiotic should not be used in monotherapy. Therefore, one of the objectives of our research was to study the efficacy of the tigecycline/rifampin combination against methicillin-resistant S. epidermidis embedded in biofilms. Of the 80 clinically significant S. epidermidis isolates, 75 strains possess the ability to form a biofilm. These bacteria formed the biofilm via ica-dependent mechanisms. However, other biofilm-associated genes, including aap (encoding accumulation-associated protein) and bhp (coding cell wall-associated protein), were present in 85 and 29 % of isolates, respectively. The biofilm structures of S. epidermidis strains were also analyzed in confocal laser scanning microscopy (CLSM) and the obtained image demonstrated differences in their architecture. In vitro studies showed that the MIC value for tigecycline against S. epidermidis growing in the biofilm ranged from 0.125 to 2 μg/mL. Tigecycline in combination with rifampin demonstrated higher activity against bacteria embedded in biofilms than tigecycline alone.

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Abbreviations

CLSM:

Confocal laser scanning microscopy

CNS:

Coagulase negative staphylococci

CRA:

Congo red agar

icaADBC:

icaADBC gene

MPU S:

Bacteriology collection of the Department of Microbiology, A. Mickiewicz University, Poznań

PBS:

Phosphate-buffered saline

PCR:

Polymerase chain reaction

PI:

Propidium iodide

PIA:

Polysaccharide intercellular adhesion

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Correspondence to Ewa Szczuka.

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Szczuka, E., Kaznowski, A. Antimicrobial activity of tigecycline alone or in combination with rifampin against Staphylococcus epidermidis in biofilm. Folia Microbiol 59, 283–288 (2014). https://doi.org/10.1007/s12223-013-0296-9

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  • DOI: https://doi.org/10.1007/s12223-013-0296-9

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