Abstract
The production of ligninolytic enzymes (laccase and Mn-dependent peroxidase) by the white-rot fungus Pleurotus pulmonarius (FR.) Quélet was studied in solid-state cultures using agricultural and food wastes as substrate. The highest activities of laccase were found in wheat bran (2,860 ± 250 U/L), pineapple peel (2,450 ± 230 U/L), and orange bagasse (2,100 ± 270 U/L) cultures, all of them at an initial moisture level of 85 %. The highest activities of Mn peroxidase were obtained in pineapple peel cultures (2,200 ± 205 U/L) at an initial moisture level of 75 %. In general, the condition of high initial moisture level (80–90 %) was the best condition for laccase activity, while the best condition for Mn peroxidase activity was cultivation at low initial moisture (50–70 %). Cultures containing high Mn peroxidase activities were more efficient in the decolorization of the industrial dyes remazol brilliant blue R (RBBR), Congo red, methylene blue, and ethyl violet than those containing high laccase activity. Also, crude enzymatic extracts with high Mn peroxidase activity were more efficient in the in vitro decolorization of methylene blue, ethyl violet, and Congo red. The dye RBBR was efficiently decolorized by both crude extracts, rich in Mn peroxidase activity or rich in laccase activity.
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Alexandrino AM, Faria HG, Souza CGM, Peralta RM (2007) Reutilisation of orange waste for production of lignocellulolytic enzymes by Pleurotus ostreatus. Cienc Technol Aliment 27:364–368
Arora DS, Sharma RK (2010) Ligninolytic fungal laccases and their biotechnological applications. Appl Biochem Biotechnol 160:1760–1788
Boer CG, Obici L, Souza CGM, Peralta RM (2004) Decolorization of synthetic dyes by solid state cultures of Lentinula (Lentinus) edodes producing manganese peroxidase as the main ligninolytic enzyme. Bioresour Technol 94:107–112
Chagas EP, Durrant LR (2001) Decolorization of azo dyes by Phanerochaete chrysosporium and Pleurotus sajor-caju. Enz Microbiol Technol 29:473–477
Cohen R, Persky L, Hadar Y (2002) Biotechnological applications and potential of wooddegrading mushrooms of the genus Pleurotus. Appl Microbiol Biotechnol 58:582–594
Couto SR (2008) Exploitation of biological wastes for the production of value-added products under solid state fermentation conditions. Biotechnol J 3:859–870
Hou H, Zhou J, Wang J, Du C, Yan B (2004) Enhancement of laccase production by Pleurotus ostreatus and its use for the decolorization of anthraquinone dye. Process Biochem 30:1415–1419
Jarosz-Wilkołazka A, Rdest-Kochmańska J, Malarczyk E, Wardas W, Leonowicz A (2002) Fungi and their ability to decolorize azo and anthraquinonic dyes. Enzyme Microbiol Technol 30:566–572
Karigar CS, Rao SS (2011) Role of microbial enzymes in the bioremediation of pollutants: a review. Enz Research. doi:10.4061/2011/805187, Article ID 805187, 11 pages
Kasinath A, Novotný C, Svobodová K, Patel KC, Sasek V (2003) Decolorisation of synthetic dyes by Irpex lacteus in liquid cultures and packed-bed bioreactor. Enzyme Microbiol Technol 32:167–173
Kuhar S, Kapoor M, Kapoor R, Sharma KK, Singh A, Kuhad RC (2007) Biodiversity of ligninolytic fungi. In: Kuhad RC, Singh A (eds) Lignocellulose biotechnology: future prospects. I. K. International, New Delhi, pp 37–53
Maciel GM, Bracht A, Souza CGM, Costa AM, Peralta RM (2012) Fundamentals, diversity and application of white-rot fungi. In: Silva AP, Sol M (eds) Fungi: types, environmental impact and role in disease. Nova Science Publishers, Inc., New York, pp 409–457
Nigam P, Armour G, Banat IM, Singh D, Marchant R (2000) Physical removal of textile dyes and solid state fermentation of dye-adsorbed agricultural residues. Bioresour Technol 72:219–226
Pointing SB, Vrjmoed LLP (2000) Decolorization of azo and triphenylmethane dyes by Picnoporus sanguineus producing laccase as the sole phenoloxidase. World J Microbiol 16:317–318
Poots VJP, McKay JJ (1976) The removal of acid dye from effluent using natural adsorbents—I peat. Water Res 10:1061–1066
Scotti CT, Vergoignan C, Feron G, Durand A (2001) Glucosamine measurement as indirect method for biomass estimation of Cunninghamella elegans grown in solid state cultivation conditions. Biochem Eng J 7:1–5
Souza CGM, Zilly A, Peralta RM (2002) Production of laccase as the sole phenoloxidase by a Brazilian strain of Pleurotus pulmonarius. J Bas Microbiol 42:83–90
Souza DF, Tychanowicz GK, Souza CGM, Peralta RM (2006) Co-production of ligninolytic enzymes by Pleurotus pulmonarius on wheat bran solid state cultures. J Bas Microbiol 46:126–134
Tychanowicz GK, Zilly A, Souza CGM, Peralta RM (2004) Decolorization of industrial dyes by solid-state cultures of Pleurotus pulmonarius. Process Biochem 39:855–859
Wariishi H, Valli K, Gold MH (1992) Manganese (II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. J Biol Chem 267:23688–23695
Willmott N, Guthrie J, Nelson G (1998) The biotechnology approach to colour removal from textile effluent. J Soc Dyers Colour 114:38–41
Zilly A, Souza CGM, Barbosa-Tessmann IP, Peralta RM (2002) Decolorisation of industrial dyes by a Brazilian strain of Pleurotus pulmonarius producing laccase as the sole phenol-oxidizing enzyme. Folia Microbiol 47:315–319
Zilly A, Bazanella GCS, Helm CV, Araújo CAV, Souza CGM, Bracht A, Peralta RM (2012) Solid-state bioconversion of passion fruit waste by white-rot fungi for production of oxidative and hydrolytic enzymes. Food Bioproc Technol 5:1573–1580
Acknowledgments
This work was supported by grants from the Fundação Araucária (PRONEX 276/08) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Edital 047/2010-SISBIOTA, proc. 563260/2010-6). R.M. Peralta, A. Bracht, D.F. Souza and R. Castoldi are fellowship holders of CNPq. R.F. Oliveira is a fellowship holder of the Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES).
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dos Santos Bazanella, G.C., de Souza, D.F., Castoldi, R. et al. Production of laccase and manganese peroxidase by Pleurotus pulmonarius in solid-state cultures and application in dye decolorization. Folia Microbiol 58, 641–647 (2013). https://doi.org/10.1007/s12223-013-0253-7
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DOI: https://doi.org/10.1007/s12223-013-0253-7