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Folia Microbiologica

, Volume 58, Issue 5, pp 375–384 | Cite as

Molecular identification and characterization of clustered regularly interspaced short palindromic repeat (CRISPR) gene cluster in Taylorella equigenitalis

  • Yasushi Hara
  • Kyohei Hayashi
  • Takuya Nakajima
  • Shizuko Kagawa
  • Akihiro Tazumi
  • John E Moore
  • Motoo MatsudaEmail author
Article

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPRs), of approximately 10,000 base pairs (bp) in length, were shown to occur in the Japanese Taylorella equigenitalis strain, EQ59. The locus was composed of the putative CRISPRs-associated with 5 (cas5), RAMP csd1, csd2, recB, cas1, a leader region, 13 CRISPR consensus sequence repeats (each 32 bp; 5′-TCAGCCACGTTCGCGTGGCTGTGTGTTTAAAG-3′). These were in turn separated by 12 non repetitive unique spacer regions of similar length. In addition, a leader region, a transposase/IS protein, a leader region, and cas3 were also seen. All seven putative open reading frames carry their ribosome binding sites. Promoter consensus sequences at the −35 and −10 regions and putative intrinsic ρ-independent transcription terminator regions also occurred. A possible long overlap of 170 bp in length occurred between the recB and cas1 loci. Positive reverse transcription PCR signals of cas5, RAMP csd1, csd2-recB/cas1, and cas3 were generated. A putative secondary structure of the CRISPR consensus repeats was constructed. Following this, CRISPR results of the T. equigenitalis EQ59 isolate were subsequently compared with those from the Taylorella asinigenitalis MCE3 isolate.

Keywords

Cluster Regularly Interspaced Short Palindromic Repeat Leader Region Short Palindromic Repeat Secondary Structure Model Extrachromosomal Element 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was partially supported by a research project grant awarded by the Azabu University (Research Services Division).

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2012

Authors and Affiliations

  • Yasushi Hara
    • 1
  • Kyohei Hayashi
    • 1
  • Takuya Nakajima
    • 1
  • Shizuko Kagawa
    • 1
  • Akihiro Tazumi
    • 1
  • John E Moore
    • 2
    • 3
    • 4
  • Motoo Matsuda
    • 1
    Email author
  1. 1.Laboratory of Molecular Biology, Graduate School of Environmental Health SciencesAzabu UniversitySagamiharaJapan
  2. 2.School of Biomedical SciencesUniversity of UlsterColeraineNorthern Ireland
  3. 3.Department of Bacteriology, Northern Ireland Public Health LaboratoryBelfast City HospitalBelfastNorthern Ireland
  4. 4.Centre for Infection and Immunity, Health Sciences BuildingQueen’s UniversityBelfastUK

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