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Lipidomic analysis of bacterial plasmalogens

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Abstract

Plasmalogens are a group of lipids with potentially important, and not yet fully known, functions in organisms from bacteria to protozoans, invertebrates, and mammals. They can protect cells against the damaging effects of reactive oxygen species, protect other phospholipids or lipoprotein particles against oxidative stress, and have been implicated as signaling molecules and modulators of membrane dynamics. They have been found in many anaerobic bacterial species, and their biosynthetic pathways differ in aerobic and anaerobic organisms. The use of advanced techniques permits the identification of not only plasmalogen classes but also their positional isomers and often also individual molecular species. This paper describes direct analyses of plasmalogens from natural sources, frequently very unusual, using electrospray ionization mass spectrometry in combination with high-performance liquid chromatography and/or shotgun lipidomics.

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Abbreviations

ACN:

Acetonitrile

AcOLi:

Lithium acetate

AcONH4 :

Ammonium acetate

CID:

Collision-induced dissociation

CL:

Cardiolipin

DHAP:

Dihydroxyacetone phosphate

ESI-MS:

Electrospray ionization mass spectrometry

EtOH:

Ethanol

FAB:

Fast atom bombardment

FAs:

Fatty acids

GC:

Gas chromatography

HILIC:

Hydrophilic interaction liquid chromatography

i-PrOH:

Propan-2-ol

IT:

Ion trap mass spectrometer

LPC:

Lysophosphatidylcholine

MALDI:

Matrix-assisted laser desorption ionization

MeOH:

Methanol

MSMA:

Magnetic sector mass-analyzer

Nano-ESI-MS-MS:

Nano-electrospray ionization tandem mass spectrometry

ND:

Not detected

PC:

Phosphatidylcholines

PE:

Phosphatidylethanolamines

PG:

Phosphatidylglycerols

PLs:

Phospholipids

Pls:

Plasmalogens

PlsPC:

Plasmalogen phosphatidylcholine

PlsPE:

Plasmalogen ethanolamines

PlsPG:

Plasmalogen phosphatidylglycerol

PlsPS:

Plasmalogen phosphatidylserine

PS:

Phosphatidylserine

Q:

Quadrupole mass spectrometer

QqQ:

Triple quadrupole mass spectrometer

QTOF:

Hybrid quadrupole time of flight mass spectrometer

ROS:

Reactive oxygen species

TIC:

Total ion current

TLC:

Thin layer chromatography

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Acknowledgments

This work was supported by project P503/11/0215 of GACR, by the Institutional Internal Project RVO61388971, and project 2B06156 of the Ministry of Education, Youth and Sports of the Czech Republic.

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 14220, Prague 4, Czech Republic

    Tomáš Řezanka, Zdena Křesinová & Karel Sigler

  2. Department of Biotechnology, Institute of Chemical Technology Prague, Technická 5, 16628, Prague, Czech Republic

    Irena Kolouchová

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  1. Tomáš Řezanka
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  2. Zdena Křesinová
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  3. Irena Kolouchová
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  4. Karel Sigler
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Correspondence to Tomáš Řezanka.

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Řezanka, T., Křesinová, Z., Kolouchová, I. et al. Lipidomic analysis of bacterial plasmalogens. Folia Microbiol 57, 463–472 (2012). https://doi.org/10.1007/s12223-012-0178-6

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