Abstract
16S rRNA sequences of ruminal uncultured bacterial clones from public databases were phylogenetically examined. The sequences were found to form two unique clusters not affiliated with any known bacterial species: cluster of unidentified sequences of free floating rumen fluid uncultured bacteria (FUB) and cluster of unidentified sequences of bacteria associated with rumen epithelium (AUB). A set of PCR primers targeting 16S rRNA of ruminal free uncultured bacteria and rumen epithelium adhering uncultured bacteria was designed based on these sequences. FUB primers were used for relative quantification of uncultured bacteria in ovine rumen samples. The effort to increase the population size of FUB group has been successful in sulfate reducing broth and culture media supplied with cellulose.
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Acknowledgments
This study was supported by the Czech Science Foundation (projects no. 525/08/H060, 523/08/1091, and P503/10/P394), National Agency for Agricultural Research of the Ministry of Agriculture of Czech Republic (project no. QI92A286/2008), and the Institutional Research Project of the Institute of Animal Physiology and Genetics, Acad. Sci. CR, v.v.i. (no. AV 0Z 5045 0515).
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Rosero, J.A., Štrosová, L., Mrázek, J. et al. PCR detection of uncultured rumen bacteria. Folia Microbiol 57, 325–330 (2012). https://doi.org/10.1007/s12223-012-0135-4
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DOI: https://doi.org/10.1007/s12223-012-0135-4
Keywords
- Uncultured Bacterium
- Rumen Fluid
- Bifidobacterium Longum
- Bovine Rumen
- Rumen Epithelium