Abstract
Strains identified in ovine cheese and bryndza by matrix-assisted laser desorption/ionization time-of-flight analysis belonged to ten species of non-enterococcal lactic acid bacteria and included Lactobacillus casei/Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus helveticus, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus brevis, Lactococcus lactis, Pediococcus pentosaceus and Pediococcus acidilactici. The susceptibility toward antibiotics was determined in lactobacilli, lactococci and pediococci and also in Escherichia coli for comparison. Analysis of L. fermentum and pediococci revealed the presence of non-wild-type epidemiological cut-offs in streptomycin, clindamycin or gentamicin. E. coli were resistant to ampicillin, tetracycline, enrofloxacin and florfenicol. No extended spectrum β-lactamases were detected.
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References
Belicová A, Križková L, Krajčovič J, Jurkovič D, Sojka M, Ebringer L, Dušinský R (2007) Antimicrobial susceptibility of Enterococcus species isolated from Slovak Bryndza cheese. Folia Microbiol 52:115–119
Berta G, Chebeňová V, Brežná B, Pangallo D, Valík L, Kuchta T (2009) Identification of lactic acid bacteria in Slovakian bryndza cheese. J Food Nutr Res 48:65–71
Chebeňová V, Berta G, Kuchta T, Brežná B, Pangallo D (2010) Randomly-amplified microsatellite polymorphism for preliminary typing of lactic acid bacteria from bryndza cheese. Folia Microbiol 55:598–602
Clark NC, Olsvik O, Swenson JM, Spiegel CA, Tenover FC (1999) Detection of a Streptomycin/Spectinomycin Adenylyltransferase Gene (aadA) in Enterococcus faecalis. Antimicrob Agents Chemother 43:157–160
Clinical Laboratory Standarts Institute (2008) Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; approved standard-third edition. CLSI document M13-A3 28:1–99, ISBN 1-56238-659-X
FEEDAP Panel (2005) Opinion of the Scientific Panel on additives and products or substances used in animal feed on the updating of the criteria used in the assessment of bacteria for resistance to antibiotics of human or veterinary importance. EFSA J 223:1–12
International Organization for Standardization (2010) ISO 10932/IDF 233 standard “Milk and milk products—determination of the minimal inhibitory concentration (MIC) of antibiotics applicable to bifidobacteria and non-enterococcal lactic acid bacteria”. 1–31
Kastner S, Perreten V, Bleuler H, Hugenschmidt G, Lacroixa C, Meile L (2006) Antibiotic susceptibility patterns and resistance genes of starter cultures and probiotic bacteria used in food. Syst Appl Microbiol 29:145–155
Klare I, Konstabel C, Werner G, Huys G, Vankerckhoven V, Kahlmeter G, Hildebrandt B, Müller-Bertling S, Witte W, Goossens H (2007) Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use. J Antimicrob Chemother 59:900–912
Kmeť V, Piatnicová E (2010) Antibiotic resistance in commensal intestinal microflora. Folia Microbiol 55:373–375
Korhonen JM, Van Hoek AHAM, Saarela M, Huys G, Tosi L, Mayrhofer S, Von Wright A (2010) Antimicrobial susceptibility of Lactobacillus rhamnosus. Benef Microbes 1:75–80
Kushiro A, Chervaux C, Cools-Portier S, Perony A, Legrain-Raspaud S, Obis D, Onoue M, van de Moer A (2009) Antimicrobial susceptibility testing of lactic acid bacteria and bifidobacteria by broth microdilution method and E-test. Int J Food Microbiol 132:54–58
Lina G, Quaglia A, Reverdy ME, Leclercq R, Vandenesch F, Etienne J (1999) Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among staphylococci. Antimicrob Agents Chemother 43:1062–1066
Mayrhofer S, van Hoek AHAM, Mair C, Huys G, Aarts HJM, Kneifel W, Domig KJ (2010) Antibiotic susceptibility of members of the Lactobacillus acidophilus group using broth microdilution and molecular identification of their resistance determinants. Int J Food Microbiol 144:81–87
SCAN (2003) Opinion of the Scientific Committee on Animal Nutrition on the criteria for assessing the safety of micro-organisms resistant to antibiotics of human clinical and veterinary importance. Available at: http://ec.europa.eu/food/fs/sc/scan/out108_en.pdf. Accessed 22 June 2011
Vakulenko SB, Donabedian SM, Voskresenskyi AM, Zervos MJ, Lerner SA, Chow JW (2003) Multiplex PCR for detection of aminoglycoside resistance genes in enterococci. Antimicrob Agents Chemother 47:1423–1426
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This work was supported by grants LPP 0045-09 and APVV 0009-10 of the Slovak Research and Development Agency.
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Kmeť, V., Drugdová, Z. Antimicrobial susceptibility of microflora from ovine cheese. Folia Microbiol 57, 291–293 (2012). https://doi.org/10.1007/s12223-012-0128-3
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DOI: https://doi.org/10.1007/s12223-012-0128-3