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Isolation, identification, and characterization of Vibrio cholerae from the Danube River in Slovakia

Folia Microbiologica volume 57, pages 191–197 (2012)Cite this article

Abstract

The occurrence of Vibrio cholerae, an important aquatic pathogen, was assessed in the surface water of the Danube River near Bratislava. The isolates were distinguished by biochemical tests and grouped by ARDRA to three clusters corresponding to three species (V. cholerae, Vibrio metschnikovii, and Aeromonas spp.). The identification of V. cholerae was confirmed by multiplex PCR using primer pairs targeted to ompW gene (membrane protein), ctxA gene (toxicity gene), and toxR gene (regulatory gene). None from the isolated V. cholerae from surface water contained ctxA gene; seven of them possessed toxR gene. Serotyping of V. cholerae isolates with polyvalent O antiserum and O/139 antiserum was negative. All isolates of V. cholerae were susceptible to chloramphenicol, rifampicin, tetracycline, variable to ampicillin, and resistant to kanamycin and streptomycin.

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Acknowledgments

This work was supported by APVV-0032-06 (Slovak Research and Developmental Agency) and VEGA 1/0117/09 (Slovak Grant Agency of Science) grants.

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Affiliations

  1. Faculty of Science, Comenius University, 842 15, Bratislava, Slovakia

    Milan Seman

  2. Water Research Institute, 812 49, Bratislava, Slovakia

    Miloslava Prokšová

  3. National Reference Center for Vibrionaceae, 945 75, Komárno, Slovakia

    Jozef Rosinský

  4. Institute of Molecular Biology, 845 51, Bratislava 45, Slovakia

    Peter Ferianc

Authors
  1. Milan Seman
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  2. Miloslava Prokšová
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  3. Jozef Rosinský
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  4. Peter Ferianc
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Correspondence to Milan Seman.

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Seman, M., Prokšová, M., Rosinský, J. et al. Isolation, identification, and characterization of Vibrio cholerae from the Danube River in Slovakia. Folia Microbiol 57, 191–197 (2012). https://doi.org/10.1007/s12223-012-0116-7

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  • DOI: https://doi.org/10.1007/s12223-012-0116-7

Keywords

  • Cholera
  • Danube Delta
  • Cholerae Strain
  • Pathogenic Vibrio
  • Alkaline Peptone Water