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Avidity of anti-neurocytoskeletal antibodies in cerebrospinal fluid and serum

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Abstract

Antibodies have different avidities that can be evaluated using modified enzyme-linked immunosorbent assay (ELISA) techniques. We determined levels and avidities of antibodies to light (NFL) and medium (NFM) subunits of neurofilaments and tau protein in serum and cerebrospinal fluid (CSF) from 26 patients and anti-tau antibody levels and their avidities in 20 multiple sclerosis (MS) patients and 20 age- and sex-matched controls. Each sample was analyzed using both standard ELISA and also using a similar ELISA protocol with the addition of urea. The avidities of anti-neurocytoskeletal antibodies were higher in the CSF than those in serum (anti-NFL, p < 0.0001; anti-tau, p < 0.01; anti-NFM, n.s.). There was no relationship between avidities in serum and CSF for individual anti-neurocytoskeletal antibodies. We did not observe the relationship among the avidities of various anti-neurocytoskeletal antibodies. The avidities of anti-tau antibodies in the CSF were significantly higher in the MS patients than those in the controls (p < 0.0001). The study demonstrates the differences in avidities of CSF or serum neurocytoskeletal antibodies measured as the urea resistance by ELISA method. Avidity determination of anti-neurocytoskeletal antibodies could contribute to the evaluation of the immunological status of patients.

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Abbreviations

BSA:

Bovine serum albumin

CSF:

Cerebrospinal fluid

ELISA:

Enzyme-linked immunosorbent assay

Ig:

Immunoglobulin

MS:

Multiple sclerosis

NFL:

The light subunit of neurofilament

NFM:

Medium subunit of neurofilament

n.s.:

Not significant

PBS:

Phosphate-buffered saline

r :

Spearman’s correlation coefficient

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Acknowledgments

The study was supported by a grant from the Ministry of Health No NS 10369-3.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. First Faculty of Medicine and General University Hospital, Institute of Medical Biochemistry and Laboratory Diagnostics, Charles University in Prague, Kateřinská 32, 121 08, Prague 2, Czech Republic

    L. Fialová, J. Švarcová & I. Malbohan

  2. Prague Psychiatric Center, Ústavní 91, 181 03, Bohnice, Prague 8, Czech Republic

    A. Bartos

  3. Third Faculty of Medicine, University Hospital Královské Vinohrady, Department of Neurology, Charles University in Prague, Šrobárova 50, 100 34, Prague 10, Czech Republic

    A. Bartos

Authors
  1. L. Fialová
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  2. J. Švarcová
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  3. A. Bartos
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  4. I. Malbohan
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Corresponding author

Correspondence to A. Bartos.

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Fialová, L., Švarcová, J., Bartos, A. et al. Avidity of anti-neurocytoskeletal antibodies in cerebrospinal fluid and serum. Folia Microbiol 57, 415–419 (2012). https://doi.org/10.1007/s12223-012-0105-x

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  • DOI: https://doi.org/10.1007/s12223-012-0105-x

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