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Studies on nutritional requirement for the culture of lichen Ramalina nervulosa and Ramalina pacifica to enhance the production of antioxidant metabolites

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Abstract

In the present report, nutritional requirement for the culture of two lichen species Ramalina nervulosa and Ramalina pacifica were studied in order to enhance their growth rate and antioxidant metabolite production. Extract of R. nervulosa cultured in Bold’s basal medium (BBM) showed higher antioxidant activity than R. pacifica cultured in Murashige and Skoog (MS) medium. The lichen species were sub-cultured in standardized nutrient media. R. nervulosa in BBM (1% glucose, 50 ppb asparagines, pH 6.5) yielded 2.76 g biomass with 26.18 mg sekikaic acid, 24.32 mg usnic acid/g dry biomass in a period of 60 days. R. pacifica in MS media (3% sucrose, 100 ppb thiamine, pH 5.9) yielded 3.54 g biomass and 58.92 mg salazinic acid, 40.16 mg usnic acid in the same time period. The standardized culture conditions implemented on bioreactor, R. nervulosa yielded 17.7 g biomass with the production of sekikaic acid 122.8 mg, usnic acid 75.4 mg in 4.5 days. R. pacifica produced 10.3 g biomass along with salazinic acid 200 mg and usnic acid 136.8 mg in the same duration. Lichen secondary metabolites produced in bioreactor showed moderate antioxidant activity; sekikaic acid 42% to 56.4%; salazinic acid 33.6% to 41.9% and usnic acid 19.9% to 29.5%.

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Acknowledgement

We gratefully acknowledge the financial support of Council of Scientific Industrial Research (grant no. 09/670 (0046) 2010/EMR-I) and Department of Biotechnology (grant no. BT/PR8551/NDB/52/15/2006), New Delhi, India. We are also thankful to Dr. BO Sharma for taxonomical identification of lichens, Dr. DG Naik, for HPLC analysis and Director, Agharkar Research Institute, Pune, for research facilities provided.

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Authors and Affiliations

  1. Mycology and Plant Pathology Group, Plant Science Division, Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, India

    Neeraj Verma & B. C. Behera

  2. School of Biochemistry, Devi Ahilya Vishwavidyalaya, Indore, 452017, India

    Ankita Joshi

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  1. Neeraj Verma
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  2. B. C. Behera
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  3. Ankita Joshi
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Correspondence to Neeraj Verma.

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Verma, N., Behera, B.C. & Joshi, A. Studies on nutritional requirement for the culture of lichen Ramalina nervulosa and Ramalina pacifica to enhance the production of antioxidant metabolites. Folia Microbiol 57, 107–114 (2012). https://doi.org/10.1007/s12223-012-0100-2

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  • DOI: https://doi.org/10.1007/s12223-012-0100-2

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