Folia Microbiologica

, Volume 56, Issue 2, pp 110–115 | Cite as

Effect of recombinant divercin RV41, structural variants and the activators of potassium channels on Listeria monocytogenes EGDe

Article

Abstract

The effect of recombinant divercin RV41 (DvnRV41) and its structural variants on the K-channel formation was determined. The growth of Listeria monocytogenes EGDe (sensitive phenotype) and its isogenic strain (resistant phenotype) was assessed in the presence of DvnRV41 combined or not with pinacidil, NS1619, cromakalim (as K-channel activators), iberiotoxin and glipizide (as K-channel blockers). The combined action of DvnRV41 and K activators permitted formation of ATP-dependent pores. The combination of DvnRV41 and ATP-dependent pore activator cromakalim inhibited the growth of sensitive strain. The antilisterial activity of structural variants was less important than that of DvnRV41 but their mode of action remained overall similar.

Abbreviations

Channel modulators

CRK

Cromakalim

GLZ

Glipizide

IBT

Iberiotoxin

NS1619

A channel opener

PCD

Pinacidil

Others

AU

Arbitrary units

Dvn RV41

Recombinant divercin

LAB

Lactic acid bacteria

LB

Luria–Bertani (broth)

MIC

Minimum inhibitory concentration

PMF

Proton motive force

PTS

Phosphotransferase system

TSB

Tryptic soy broth

TSYEB

TSB + yeast extract

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Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2011

Authors and Affiliations

  1. 1.Food Science and Engineering, La ChantrerieNantes-Atlantic National College of Veterinary MedicineNantes Cedex 03France
  2. 2.Department of Biochemistry and MicrobiologyInstitute of Chemical TechnologyPrague 6Czech Republic

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