Abstract
The iol cluster (consisting of genes involved in myo-inositol utilization) was investigated in Lactobacillus casei strains isolated from koumiss. Ten strains were tested for the presence of iol cluster by PCR screening; three strains encoded this cluster. Full-sequencing procedure was conducted; the iol cluster was identical to that of L. casei BL23 (GenBank access. no. FM177140) except for an upstream transposase. The iol cluster is not a common feature for L. casei strains isolated from koumiss.
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Abbreviations
- mIol:
-
myo-inositol
- LAB:
-
lactic acid bacteria
- MRS:
-
de Man-Rogosa-Sharpe (broth)
References
Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J.: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl.Acids Res.25, 3389–3402 (1997).
Besemer J., Lomsadze A., Borodovsky M.: GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Nucl.Acids Res.29, 2607–2618 (2001).
Bordenstein S.R., Reznikoff W.S.: Mobile DNA in obligate intracellular bacteria. Nat.Rev.Microbiol.3, 688–699 (2005).
Brown L.D., Cheung A., Harwood J.E., Battaglia F.C.: Inositol and mannose utilization rates in term and late-preterm infants exceed nutritional intakes. J.Nutr.139, 1648–1652 (2009).
Ewing B., Green P.: Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res.8, 186–194 (1998).
Fujita Y., Shindo K., Miwa Y., Yoshida K.: Bacillus subtilis inositol dehydrogenase-encoding gene (idh): sequence and expression in Escherichia coli. Gene108, 121–125 (1991).
Galbraith M.P., Feng S.F., Borneman J., Triplett E.W., De Bruijn F.J., Rossbach S.: A functional myo-inositol catabolism pathway is essential for rhizopine utilization by Sinorhizobium meliloti. Microbiology (Reading, England)144, 2915–2924 (1998).
Heller K.J.: Probiotic bacteria in fermented foods: product characteristics and starter organisms. Am.J.Clin.Nutr.73, 374S–379S (2001).
Hasisurong, Amuguleng, Manglai: Koumiss and its value in medicine. Zhongguo Zhong Yao Za Zhi28, 11–14 (2003).
Holub B.J.: Metabolism and function of myo-inositol and inositol phospholipids. Ann.Rev.Nutr.6, 563–597 (1986).
Ishii S., Kikuchi M., Muramatsu K., Takao S.: Identification of compounds causing symbiotic growth of Lactobacillus paracasei subsp. tolerans and Kluyveromyces marxianus var. lactis (Chigo, Inner Mongolia, China). Anim.Sci.J.70, 81–89 (1999).
Jauniaux E., Hempstock J., Teng C., Battaglia F.C., Burton G.J.: Polyol concentrations in the fluid compartments of the human conceptus during the first trimester of pregnancy: maintenance of redox potential in a low oxygen environment. J.Clin. Endocrinol.Metab.90, 1171–1175 (2005).
Jiang G., Krishnan A.H., Kim Y.W., Wacek T.J., Krishnan H.B.: A functional myo-inositol dehydrogenase gene is required for efficient nitrogen fixation and competitiveness of Sinorhizobium fredii USDA191 to nodulate soybean (Glycine max [L.]Merr.). J.Bacteriol.183, 2595–2604 (2001).
Krings E., Krumbach K., Bathe B., Kelle R., Wendisch V.F., Sahm H., Eggeling L.: Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. J.Bacteriol. 188, 8054–8061 (2006).
Kurtz S., Choudhuri J.V., Ohlebusch E., Schleiermacher C., Stoye J., Giegerich R.: REPuter: the manifold applications of repeat analysis on a genomic scale. Nucl.Acids Res.29, 4633–4642 (2001).
Loewus F.A., Loewus M.W.: myo-Inositol: its biosynthesis and metabolism. Ann.Rev.Plant Physiol.34, 137–161 (1983).
Molenaar D., Bringel F., Schuren F.H., De Vos W.M., Siezen R.J., Kleerebezem M.: Exploring Lactobacillus plantarum genome diversity by using microarrays. J.Bacteriol.187, 6119–6127 (2005).
Molina Y., Ramos S.E., Douglass T., Klig L.S.: Inositol synthesis and catabolism in Cryptococcus neoformans, pp. 1657–1667 in Yeast, Vol. 15. Chichester, England 1999.
Parvez S., Malik K.A., Ah Kang S., Kim H.Y.: Probiotics and their fermented food products are beneficial for health. J.Appl.Microbiol.100, 1171–1185 (2006).
Reid G.: The importance of guidelines in the development and application of probiotics. Curr.Pharm.Des.11, 11–16 (2005).
de Vos W.M., Hugenholtz J.: Engineering metabolic highways in lactococci and other lactic acid bacteria. Trends Biotechnol.22, 72–79 (2004).
Watanabe K., Fujimoto J., Sasamoto M., Dugersuren J., Tumursuh T., Demberel S.: Diversity of lactic acid bacteria and yeasts in Airag and Tarag, traditional fermented milk products of Mongolia. World J.Microbiol.Biotechnol.24, 1313–1325 (2008).
Wang J., Chen X., Liy W., Yang M., Zhang H.: Identification of Lactobacillus from koumiss by conventional and molecular methods. Eur.Food Res.Technol.227, 1555–1561 (2008).
Yebra M.J., Zuniga M., Beaufils S., Perez-martinez G., Deutscher J., Monedero V.: Identification of a gene cluster enabling Lactobacillus casei BL23 to utilize myo-inositol. Appl.Environ.Microbiol.73, 3850–3858 (2007).
Yoshida K., Yamaguchi M., Morinaga T., Kinehara M., Ikeuchi M., Ashida H., Fujita Y.: myo-Inositol catabolism in Bacillus subtilis. J.Biol.Chem.283, 10415–10424 (2008).
Zhang W., Yun Y., Sun T., Menghe B., Zhang H.: Isolation and identification of dominant microorganisms involved in naturally fermented goat milk in Haixi region of Qinghai, China. Ann.Microbiol.58, 213–217 (2008).
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Zhang, W., Sun, Z., Sun, T. et al. PCR screening and sequence analysis of iol clusters in Lactobacillus casei strains isolated from koumiss. Folia Microbiol 55, 603–606 (2010). https://doi.org/10.1007/s12223-010-0097-3
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DOI: https://doi.org/10.1007/s12223-010-0097-3