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Bacterial community composition of the gut microbiota of Cylindroiulus fulviceps (diplopoda) as revealed by molecular fingerprinting and cloning

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Abstract

Bacterial clone libraries of the gut microbiota of nurtured and starved Cylindroiulus fulviceps specimens displayed the predominance of the phyla Bacteroidetes (55 and 37 %, respectively) and Proteobacteria (40 and 35 %, respectively) and a high similarity to bacteria previously detected in the intestinal tract of termites and beetles, which are known to harbor symbiotic bacteria essential for digestive activity. Bacterial isolates were dominated by Proteobacteria (74 %), followed by members of the phyla Actinobacteria, Firmicutes and Bacteroidetes. PCR-DGGE fingerprints of the gut samples showed that intestinal bacteria were affected by starvation, although the change was not significant.

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Abbreviations

DGGE:

denaturing gradient gel electrophoresis

ICD:

intestines of diplopods fed with cow dung

ISA:

intestines of starved diplopods

OTUs:

operational taxonomic units

PCO:

principal coordinate analysis

RDP:

ribosomal database project

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Authors and Affiliations

  1. Institute of Microbiology, University of Innsbruck, 6020, Innsbruck, Austria

    B. A. Knapp & H. Insam

  2. Institute of Ecology, University of Innsbruck, 6020, Innsbruck, Austria

    J. Seeber, A. Rief & E. Meyer

Authors
  1. B. A. Knapp
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  2. J. Seeber
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  3. A. Rief
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  4. E. Meyer
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  5. H. Insam
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Correspondence to B. A. Knapp.

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Knapp, B.A., Seeber, J., Rief, A. et al. Bacterial community composition of the gut microbiota of Cylindroiulus fulviceps (diplopoda) as revealed by molecular fingerprinting and cloning. Folia Microbiol 55, 489–496 (2010). https://doi.org/10.1007/s12223-010-0081-y

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  • DOI: https://doi.org/10.1007/s12223-010-0081-y

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