Folia Microbiologica

, Volume 55, Issue 3, pp 277–280 | Cite as

Impact of heat-inactivated Lactobacillus casei and Lactobacillus paracasei strains on cytokine responses in whole blood cell cultures of children with atopic dermatitis

  • B. Cukrowska
  • I. Rosiak
  • E. Klewicka
  • I. Motyl
  • M. Schwarzer
  • Z. Libudzisz
  • H. Kozakova
Article

Abstract

Heat-inactivated Lactobacillus casei LOCK 0900, L. casei LOCK 0908 and Lactobacillus paracasei LOCK 0919 strains, applied to blood cell cultures obtained from children with atopic dermatitis induced production of anti-allergic TH1 cytokines (interleukin-12, interleukin-18, interferon-γ, tumor necrosis factor-α) and regulatory transforming growth factor-β1), but did not stimulate pro-allergic interleukin-5. The lactobacilli-mixture remarkably enhanced the TH1 response compared to single strains. This synergistic effect was not observed for transforming growth factor-β1. In contrast, the amount of interleukin-10 was found to be considerably lower when cells were stimulated with lactobacilli-mixture compared to single strains. The mixture of Lactobacillus strains represents a probiotic bacterial preparation modulating in vitro cytokine profile of allergic children towards anti-allergic TH1 response.

Abbreviations

IFN

interferon

IL

interleukin

PBS

phosphate-buffered saline

PHA

phytohemagglutinin

TGF

transforming growth factor

TH

T helper (lymphocytes)

TNF

tumor necrosis factor

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Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2010

Authors and Affiliations

  • B. Cukrowska
    • 1
  • I. Rosiak
    • 1
  • E. Klewicka
    • 2
  • I. Motyl
    • 2
  • M. Schwarzer
    • 3
  • Z. Libudzisz
    • 2
  • H. Kozakova
    • 3
  1. 1.Department of PathologyThe Children’s Memorial Health InstituteWarsawPoland
  2. 2.Institute of Fermentation Technology and MicrobiologyTechnical University of LodzLodzPoland
  3. 3.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicNovy HradekCzech Republic

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