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Genotyping and PCR detection of potential virulence genes in Campylobacter jejuni and Campylobacter coli isolates from different sources in Poland

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Abstract

The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was detected by PCR. High prevalence of five different putative virulence and toxin genes (flaA, cdtA, cdtB, cdtC, and cj0588) was found among isolates obtained from children, chickens and dogs. The occurrence of these genes among isolates obtained from pigs was significantly different than for strains isolated from other sources. Two methods for genotyping Campylobacter spp. strains were applied — flaA-typing, and ADSRRS-fingerprinting method, which was used for the first time for Campylobacter spp. strains. Similarity of the genetic profiles was demonstrated in strains isolated from chickens and dogs, and in isolates from chickens and children. Strains isolated from pigs, both C. jejuni as well as C. coli, did not group with isolates from other sources.

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Abbreviations

ADSRRS:

amplification of DNA fragments surrounding rare restriction sites

CDT:

cytolethal distending toxin

CDT:

cytolethal distending toxin

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Authors and Affiliations

  1. Department of Pre-Clinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-786, Warsaw, Poland

    A. Krutkiewicz & D. Klimuszko

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  1. A. Krutkiewicz
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  2. D. Klimuszko
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Correspondence to A. Krutkiewicz.

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Krutkiewicz, A., Klimuszko, D. Genotyping and PCR detection of potential virulence genes in Campylobacter jejuni and Campylobacter coli isolates from different sources in Poland. Folia Microbiol 55, 167–175 (2010). https://doi.org/10.1007/s12223-010-0025-6

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  • DOI: https://doi.org/10.1007/s12223-010-0025-6

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