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DRBC agar: a new tool for Candida dubliniensis identification

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Abstract

Candida dubliniensis pathogenic species, which shares many phenotypic features with C. albicans, may be misidentified in the microbiology laboratory. The growth on DRBC agar at 25 °C was shown to be a new tool for differentiation between C. dubliniensis and C. albicans. All 27 isolates of C. dubliniensis showed in this medium rough colonies (peripheral hyphal fringes) and abundant chlamydospore production, while all 103 isolates of C. albicans showed smooth colonies without fringes or chlamydospores. DRBC agar allowed the differentiation of C. albicans from C. dubliniensis with 100 % sensitivity and specificity.

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Abbreviations

DRBC:

dichloran-Rose Bengal-chloramphenicol (medium)

HIV:

human immunodeficiency virus

FN:

false negative (results)

FP:

false positive

TN:

true negative

TP:

true positive

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Authors and Affiliations

  1. Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria (RS), 97010-033, Brazil

    L. Alves Scheid, D. A. Nunes Mario, I. H. Soares, É. Silva Loreto, J. M. Santurio & S. Hartz Alves

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  1. L. Alves Scheid
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  2. D. A. Nunes Mario
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  3. I. H. Soares
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  4. É. Silva Loreto
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  5. J. M. Santurio
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  6. S. Hartz Alves
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Correspondence to S. Hartz Alves.

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Scheid, L.A., Nunes Mario, D.A., Soares, I.H. et al. DRBC agar: a new tool for Candida dubliniensis identification. Folia Microbiol 55, 155–158 (2010). https://doi.org/10.1007/s12223-010-0023-8

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  • DOI: https://doi.org/10.1007/s12223-010-0023-8

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