Abstract
An incomplete oligoketide (PK; ‘polyketide’) gene cluster, aur1, responsible for the production of an angucycline-like antibiotic auricin was identified in Streptomyces aureofaciens CCM 3239. A region downstream of the aur1 was cloned and sequenced, revealing 28 new genes encoding putative protein products involved in deoxysugar biosynthesis and other putative PK-related biosynthetic functions. In addition, a gene, bpsA, encoding a protein similar to non-ribosomal peptide synthetases (NRPSs) was identified in this region. A deduced protein product of the gene showed the highest similarity to NRPSs IndC from Erwinia chrysanthemi and BpsA from Streptomyces lavendulae, both involved in the biosynthesis of a blue pigment indigoidine. S. aureofaciens CCM 3239 was found to produce an extracellular blue pigment with identical properties as indigoidine. A deletion mutant of bpsA in S. aureofaciens CCM 3239 failed to produce the blue pigment. In addition, the deletion of bpsA had a positive effect on auricin production. The results indicate the involvement of the bpsA gene in biosynthesis of the indigoidine blue pigment in S. aureofaciens CCM 3239.
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Abbreviations
- CFU:
-
colony forming unit(s)
- DIG:
-
digoxygenin
- DMF:
-
dimethylformamide
- DMSO:
-
dimethylsulfoxid
- NMP:
-
N-methylpyrrolidone
- NRPS:
-
non-ribosomal peptide synthetases
- ORF:
-
open reading frame
- PCR:
-
polymerase chain reaction
- PK:
-
oligoketide (‘polyketide’)
- PKS:
-
oligoketide (‘polyketide’) synthase
- THF:
-
tetrahydrofurane
- WT:
-
wild type
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Novakova, R., Odnogova, Z., Kutas, P. et al. Identification and characterization of an indigoidine-like gene for a blue pigment biosynthesis in Streptomyces aureofaciens CCM 3239. Folia Microbiol 55, 119–125 (2010). https://doi.org/10.1007/s12223-010-0018-5
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DOI: https://doi.org/10.1007/s12223-010-0018-5