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High level of quinolone resistance in Escherichia coli from healthy chicken broilers

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Abstract

The development of resistance to quinolones (nalidixic acid, ciprofloxacin and enrofloxacin) in 2006–2008 was evaluated in 317 strains of Escherichia coli isolated from healthy chicken broilers from various farms. The isolates (2006/2007/2008) showed a high resistance to nalidixic acid (87/85/67 %), ciprofloxacin (CIP) (49/54/29 %) and enrofloxacin (ENR) (52/42/22 %). Nalidixic acid-resistant isolates with low level of MIC for CIP and ENR represented a single mutation; intermediary MIC for CIP and ENR were related to two mutations and high level resistance MIC for CIP (≥4 mg/L) and ENR (≥16 mg/L) represented three mutations (two in gyrA and one in parC). There was a correlation between the phenotype reading of high-level resistance and mutations in gyrA (Ser83Leu, Asp87Tyr or Asp87Asn) and parC (Ser80Ile) gene. Plasmid-mediated quinolone-resistance qnrS gene was detected in one Escherichia coli strain with a high level of ciprofloxacin resistance. Our results demonstrate the increase in occurrence of multiresistant E. coli strains with a high level of chromosomal and plasmid resistance to fluoroquinolones.

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Abbreviations

AMP:

ampicillin

CIP:

ciprofloxacin

CMP:

chloramphenicol

COT:

cotrimoxazol

ENR:

enrofloxacin

GEN:

gentamicin

NEO:

neomycin

SPE:

spectinomycin

STM:

streptomycin

TTC:

tetracycline

Asn:

asparagine

Asp:

aspartic acid

Ile:

isoleucine Ser serine

Leu:

leucine

Ser:

serine

Tyr:

tyrosine

CLSI:

Clinical Laboratory Standards Institute

ESBL(s):

extended spectrum β-lactamase(s)

MICxG:

geometric mean of MIC

PMQR:

plasmid mediated quinolone resistance

QRDR:

quinolone-resistance determining region

References

  • Belicová A., Križková L., Krajčovič J., Jurkovič D., Sojka M., Ebringer L., Dušinský R.: Antimicrobial susceptibility of Enterococcus species isolated from Slovak Bryndza cheese. Folia Microbiol. 52, 115–119 (2007).

    Article  Google Scholar 

  • EFSA: Scientific opinion of the Panel on Biological Hazards on a request from the European Food Safety Authority on foodborne antimicrobial resistance as a biological hazard. EFSA J. 765, 1–87 (2008)

    Google Scholar 

  • EMEA report: Public statement on the use of (fluoro)quinolones in food-producing animals in the European Union, pp. 1–24 in Report of European Medicines Agency, London 2007.

  • Everett M.J., Jin Y.F., Ricci V., Piddock L.J.: Contribution of individual mechanisms to fluoroquinolone resistance in 36 Escherichia coli strains isolated from humans and animals. Antimicrob.Agents Chemother. 40, 2380–2386 (1996)

    CAS  PubMed  Google Scholar 

  • Giraud E., Leroy-Setrin S., Flaujac G., Cloeckaert A., Dho-Moulin M., Chaslus-Dancla E.: Characterization of high-level fluoroquinolone resistance in Escherichia coli O78:K80 isolated from turkeys. J.Antimicrob.Chemother.47, 341–343 (2001).

    Article  CAS  PubMed  Google Scholar 

  • Guerra B., Junker E., Schroeter A., Malorny B., Lehmann S., Helmuth R.: Phenotypic and genotypic characterization of antimicrobial resistance in German Escherichia coli isolates from cattle, swine and poultry. J.Antimicrob.Chemother.52, 489–492 (2003).

    Article  CAS  PubMed  Google Scholar 

  • Kehrenberg C., de Jong A., Friederichs S., Cloeckaert A., Schwarz S.: Molecular mechanisms of decreased susceptibility to fluoroquinolones in avian Salmonella serovars and their mutants selected during the determination of mutant prevention concentrations. J.Antimicrob.Chemother.59, 886–892 (2007).

    Article  CAS  PubMed  Google Scholar 

  • Kolář M., Bardoň J., Sauer P., Kesselová M., Čekanová L., Vágnerová I., Koukalová D., Hejnar P.: Fluoroquinolone-resistant Escherichia coli and Proteus mirabilis in poultry of middle Moravia, Czech Republic. Acta Vet.Brno74, 249–253 (2005).

    Google Scholar 

  • Lee Y.J., Cho J.K., Kim K.S., Tak R.B., Kim A.R., Kim J.W., Im S.K., Kim H.: Fluoroquinolone resistance and gyrA and parC mutations of Escherichia coli isolated from chicken. J.Microbiol43, 391–397 (2005).

    CAS  PubMed  Google Scholar 

  • Ma J., Zeng Z., Chen Z., Xu X., Wang X., Deng Y., Lü D., Huang L., Zhang Y., Liu J., Wang M.: High prevalence of plasmid-mediated quinolone resistance determinants qnr, aac(6′)-Ib-cr, and qepA among ceftiofur-resistant Enterobacteriaceae isolates from companion and food-producing animals. Antimicrob.Agents Chemother.53, 519–524 (2009).

    Article  CAS  PubMed  Google Scholar 

  • Morgan-Linnell S.K., Boyd L.B., Steffen D., Zechiedrich L.: Mechanisms accounting for fluoroquinolone resistance in Escherichia coli clinical isolates. Antimicrob.Agents Chemother.53, 235–242 (2009).

    Article  CAS  PubMed  Google Scholar 

  • Perichon B., Courvalin P., Galimand M.: Transferable resistance to aminoglycosides by methylation of G1405 in 16S rRNA and to hydrophilic fluoroquinolones by QepA-mediated efflux in Escherichia coli. Antimicrob.Agents Chemother.51, 2464–2469 (2007).

    Article  CAS  PubMed  Google Scholar 

  • Robicsek A., Sahm D.F., Strahilevitz J., Jacoby G.A., Hooper D.C.: Broader distribution of plasmid-mediated quinolone resistance in the United States. Antimicrob.Agents Chemother.49, 3001–3003 (2005).

    Article  CAS  PubMed  Google Scholar 

  • Robicsek A., Strahilevitz J., Sahm D.F., Hooper D.C.: qnr prevalence in ceftazidime-resistant Enterobacteriaceae isolates from the United States. Antimicrob.Agents Chemother.50, 2872–2874 (2006).

    Article  CAS  PubMed  Google Scholar 

  • Taylor N.M., Davies R.H., Ridley A., Clouting C., Wales A.D., Clifton-Hadley F.A.: A survey of fluoroquinolone resistance in Escherichia coli and thermophilic Campylobacter spp. on poultry and pig farms in Great Britain. J.Appl.Microbiol.105, 1421–1431 (2008).

    Article  CAS  PubMed  Google Scholar 

  • Wojnicz D., Klak M., Adamski R., Jankowski S.: Influence of subinhibitory concentrations of amikacin and ciprofloxacin on morphology and adherence ability of uropathogenic strains. Folia Microbiol.52, 429–436 (2007).

    Article  CAS  Google Scholar 

  • Yamane K., Wachino J., Suzuki S., Arakawa Y.: Plasmid-mediated qepA gene among Escherichia coli clinical isolates from Japan. Antimicrob.Agents Chemother.52, 1564–1566 (2008).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Animal Physiology, Slovak Academy of Sciences, Košice, 040 01, Slovakia

    V. Kmeť

  2. Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine, University of P.J. Šafárik, 040 01, KoŠice, Slovakia

    M. Kmeťová

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  1. V. Kmeť
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  2. M. Kmeťová
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Correspondence to V. Kmeť.

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Kmeť, V., Kmeťová, M. High level of quinolone resistance in Escherichia coli from healthy chicken broilers. Folia Microbiol 55, 79–82 (2010). https://doi.org/10.1007/s12223-010-0013-x

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  • DOI: https://doi.org/10.1007/s12223-010-0013-x

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