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Susceptibility of Staphylococcus aureus strains toward combinations of oxacillin-2,4-dihydroxychalcone

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Abstract

In order to determine the existence of synergism of the bacteriostatic action of flavonoids against G+ bacteria between a clinically interesting conventional antibiotic and a flavonoid, combinations of oxacillin (OXC) and 2,4-dihydroxychalcone (DCH) as enhancer were assayed against methicillin-sensitive Staphylococcus aureus ATCC 29 213 and methicillin-resistant S. aureus ATCC 43 300. Using a kinetic-turbidimetric method, growth kinetics was monitored in a broth containing variable amounts of OXC alone and combinations of variable OXC-constant DCH. The minimum inhibitory concentrations (MIC) of OXC alone and in combination with DCH were evaluated. For the 29 213 strain, OXC MIC was 25 μg/mL, while combinations of 2–8 μg/mL OXC with 10 μg/mL of DCH totally inhibited growth and showed synergism. The resistance of the 43 300 strain in the presence of OXC was verified; OXC-DCH combinations decreased bacterial growth by 35 %. DCH augments the action of OXC against methicillin-susceptible S. aureus and therefore constitutes a good bacteriostatic agent for methicillin-resistant S. aureus.

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Abbreviations

DCH:

2,4-dihydroxychalcone

MIC:

minimum inhibitory concentration

MRSA:

methicillin-resistant (S. aureus)

MSSA:

methicillin-sensitive (S. aureus)

OXC:

oxacillin

PRSP:

penicillin-resistant (S. pneumoniae)

VRE:

vancomycin-resistant enterococci

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Authors and Affiliations

  1. Laboratory of Physical Chemistry, Department of Chemistry, San Luis National University, 5700, San Luis, Argentina

    J. M. Talia, M. A. Alvarez, N. B. Debattista & N. B. Pappano

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  1. J. M. Talia
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  2. M. A. Alvarez
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  3. N. B. Debattista
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  4. N. B. Pappano
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Correspondence to N. B. Pappano.

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Talia, J.M., Alvarez, M.A., Debattista, N.B. et al. Susceptibility of Staphylococcus aureus strains toward combinations of oxacillin-2,4-dihydroxychalcone. Folia Microbiol 54, 516–520 (2009). https://doi.org/10.1007/s12223-009-0074-x

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  • DOI: https://doi.org/10.1007/s12223-009-0074-x

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