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N-terminal anchor in surface immunogenic protein of Streptococcus agalactiae and its influence on immunity elicitation

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Abstract

A multiplex PCR method was developed to characterize the potential variability within the sip gene from bovine isolates of Streptococcus agalactiae. An incomplete sip gene (ncosip) was found in four isolates of S. agalactiae. According to the in silico analysis of the missing region at amino acid level, the N-terminal of surface immunogenic protein (Sip) was found to contain a LysM domain motif; whilst the incomplete Sip (NcoSip) lacked a part of this motif. Immunity elicitation against Sip was confirmed by immunization of mice. This response was partially observed also with NcoSip.

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Abbreviations

ELISA:

enzyme-linked immunosorbent assay

PBS:

phosphate-buffered saline

LB:

Luria-Bertani

Sip:

surface immunogenic protein

NcoSip:

incomplete Sip protein

SSS:

sip specific sequence

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Authors and Affiliations

  1. Institute of Biochemistry, Nutrition and Health Protection, Slovak Technical University, 812 37, Bratislava, Slovakia

    B. Vidová

  2. Institute of Molecular Biology, Slovak Academy of Sciences, 845 51, Bratislava, Slovakia

    B. Vidová, M. Chotár & A. Godány

Authors
  1. B. Vidová
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  2. M. Chotár
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  3. A. Godány
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Correspondence to B. Vidová.

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Vidová, B., Chotár, M. & Godány, A. N-terminal anchor in surface immunogenic protein of Streptococcus agalactiae and its influence on immunity elicitation. Folia Microbiol 54, 161–166 (2009). https://doi.org/10.1007/s12223-009-0025-6

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  • DOI: https://doi.org/10.1007/s12223-009-0025-6

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