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Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis

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Abstract

Using two primer pairs constructed in silico for the amplification of the intervening sequences (IVSs) of the 23S rRNA gene sequences of the genus Taylorella, none of the three representative T. equigenitalis strains NCTC11184T, Kentucky 188 and EQ59 was shown to contain any IVSs in the first quarter region. In the central region, all three strains possessed one ≈70 bp IVS (TeIVS2) different from any IVSs found in T. asinigenitalis. The predicted secondary structure model of the IVSs contained stem and loop structures. The central region of the IVS-stem structure contains an identical double-stranded consensus 15-bp sequence. The purified RNA fraction from the three strains contained 16S and 4-5S RNA species but no 23S rRNA species. Thus, the primary 23S rRNA transcripts from the three strains would be cleaved into approximately 1.2- and 1.6-kb rRNA fragments and ≈70-bp IVS. In addition, 16 other T. equigenitalis isolates were found to carry a similar 70-bp IVS in the central region and to produce fragmented 23S rRNA.

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Abbreviations

CEM:

contagious equine metritis

IVS(s):

intervening sequence(s)

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Authors and Affiliations

  1. Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Fuchinobe 1-17-71, Sagamihara, 229-8501, Japan

    A. Tazumi, T. Sekizuka, I. Taneike & M. Matsuda

  2. Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast, BT9 7AD, Northern Ireland, UK

    J. E. Moore & B. C. Millar

Authors
  1. A. Tazumi
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  2. T. Sekizuka
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  3. J. E. Moore
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  4. B. C. Millar
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  5. I. Taneike
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  6. M. Matsuda
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Correspondence to M. Matsuda.

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Tazumi, A., Sekizuka, T., Moore, J.E. et al. Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis . Folia Microbiol 53, 486–492 (2008). https://doi.org/10.1007/s12223-008-0076-0

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  • DOI: https://doi.org/10.1007/s12223-008-0076-0

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