Skip to main content
Log in

Ecological and physiological characterization shows that Fibrobacter succinogenes is important in rumen fiber digestion — Review

  • Published:
Folia Microbiologica Aims and scope Submit manuscript


Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digestion. These data indicate that group 1 should be the focus of future efforts to maximize the fibrolytic function of the rumen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others



denatured gradient gel electrophoresis

FISH (method):

fluorescence in situ hybridization (method)


polymerase chain reaction


quantitative PCR


restriction fragment length polymorphism


  • Amann R.L., Lin C., Key R., Montgomery L., Stahl D.A.: Diversity among Fibrobacter isolates: towards a phylogenetic classification. Syst.Appl.Microbiol.15, 23–31 (1992).

    Google Scholar 

  • Be’ra-Maillet C., Ribot Y., Forano E.: Fiber-degrading systems of different strains of the genus Fibrobacter. Appl.Environ.Microbiol.70, 2172–2179 (2004).

    Article  CAS  Google Scholar 

  • Cheng K.J., Stewart C.S., Dinsdale D., Costerton J.W.: Electron microscopy of bacteria involved in the digestion of plant cell walls. Anim.Feed Sci.Technol.10, 93–120 (1984).

    Article  Google Scholar 

  • Fields M.W., Mallik S., Russell J.B.: Fibrobacter succinogenes S85 ferments ball-milled cellulose as fast as cellobiose until cellulose surface area is limiting. Appl.Environ.Microbiol.54, 570–574 (2000).

    CAS  Google Scholar 

  • Jun H.S., Qi M., Ha J.K., Forsberg C.W.: Fibrobacter succinogenes, a dominant fibrolytic ruminal bacterium: transition to the post genomic era. Asian-Austral.J.Anim.Sci.20, 802–810 (2007).

    CAS  Google Scholar 

  • Koike S., Kobayashi Y.: Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiol.Lett.204, 361–366 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Koike S., Pan J., Kobayashi Y., Tanaka K.: Kinetics of in sacco fiber-attachment of representative ruminal cellulolytic bacteria monitored by competitive PCR. J.Dairy Sci.86, 1429–1435 (2003).

    PubMed  CAS  Google Scholar 

  • Koike S., Pan J., Suzuki T., Takano T., Oshima C., Kobayashi Y., Tanaka K.: Ruminal distribution of the cellulolytic bacterium Fibrobacter succinogenes in relation to its phylogenetic grouping. Anim.Sci.J.75, 417–422 (2004).

    Article  CAS  Google Scholar 

  • Koike S., Yabuki H., Kobayashi Y.: Validation and application of real-time PCR assays for representative rumen bacteria. Anim.Sci.J.78, 135–141 (2007).

    Article  CAS  Google Scholar 

  • Kopečný J., Hodrová B.: The effect of yellow affinity substance on cellulases of Ruminococcus flavefaciens. Lett.Appl.Microbiol.25, 191–196 (1997).

    Article  PubMed  Google Scholar 

  • Kozakai K., Nakamura T., Kobayashi Y., Tanigawa T., Osaka I., Kawamoto S., Hara S.: Effect of mechanical processing of corn silage on bacterial colonization and fermentation in the rumen. Can.J.Anim.Sci.87, 259–267 (2007).

    Google Scholar 

  • Lin C., Flesher B., Capman W.C., Amann R.I., Stahl D.A.: Taxon-specific hybridization probes for fiber-digesting bacteria suggest novel gut-associated Fibrobacter. Syst.Appl.Microbiol.17, 418–424 (1994).

    CAS  Google Scholar 

  • Matulova M., Noualle R., Capek P., Pe’an M., Forano E., Delfort A.M.: Degradation of wheat straw by Fibrobacter succinogenes S85: a liquid and solid-state nuclear magnetic resonance study. Appl.Environ.Microbiol.71, 1247–1253 (2005).

    Article  PubMed  CAS  Google Scholar 

  • Michalet-Doreau B., Fernandez I., Peyron C., Millet L., Fonty G.: Fibrolytic activities and cellulolyitc bacterial community structure in the solid and liquid phases of rumen contents. Reprod.Nutr.Dev.41, 187–194 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Michalet-Doreau B., Fernandez I., Fonty G.: A comparison of enzymatic and molecular approaches to characterize the cellulolytic microbial ecosystems of the rumen and the cecum. J.Anim.Sci.80, 790–796 (2002).

    PubMed  CAS  Google Scholar 

  • Mirobn J., Ben-Ghedallia D.: The degradation and utilization of monosaccharide components by defined ruminal cellulolytic bacteria. Appl.Microbiol.Biotechnol.38, 432–437 (1992).

    Google Scholar 

  • Mosoni P., Chaucheyras-Durrand F., Bera-Maillet C., Forano E.: Quantification by real-time PCR of cellulolytic bacteria in the rumen of sheep after supplementation of a forage diet with readily fermentable carbohydrates: effect of a yeast additive. J.Appl.Microbiol., in press (2008).

  • Rogar V., Fonty G., Komisarezuk-Bony S., Gouet P.: Effect of physicochemical factors on the adhesion to cellulose Avicel of the ruminal bacteria Ruminococcus flavefaciens and Fibrobacter succinogenes subsp. succinogenes. Appl.Environ.Microbiol.56, 3071–3087 (1990).

    Google Scholar 

  • Saluzzi L., Smith A., Stewart C.S.: Analysis of bacterial phospholipid markers and plant monosaccharides during forage degradation by Ruminococcus flavefaciens and Fibrobacter succinogenes in co-culture. J.Gen.Microbiol.139, 2865–2873 (1993).

    PubMed  CAS  Google Scholar 

  • Sawanon S., Shinkai T., Koike S., Kobayashi Y., Tanaka K.: Indication of a novel group of Selenomonas ruminantium with high cellulase and fiber-attaching activities from the rumen, pp. 363–368 in K. Ohmiya, K. Sakka, S. Karita, T. Kimura, M. Sakka, Y. Ohnishi (Eds): Biotechnology of Lignocellulose Degradation and Biomass Utilization. Uni Publishers, Tokyo 2003.

    Google Scholar 

  • Sawanon S., Kobayashi Y.: Synergistic fibrolysis in the rumen by cellulolytic Ruminococcus flavefaciens and non-cellulolytic Selenomonas ruminantium: evidence in defined cultures. Anim.Sci.J.77, 208–214 (2006).

    Article  CAS  Google Scholar 

  • Shinkai T., Kobayashi Y.: Localization of ruminal cellulolytic bacteria on plant fibrous materials as determined by fluorescent in situ hybridization and real-time PCR. Appl.Environ.Microbiol.73, 1646–1652 (2007).

    Article  PubMed  CAS  Google Scholar 

  • Shinkai T., Matsumoto N., Kobayashi Y.: Ecological characterization of three different phylogenetic groups belonging to the cellulolytic bacterial species Fibrobacter succinogenes in the rumen. Anim.Sci.J.78, 503–511 (2007).

    Article  CAS  Google Scholar 

  • Stewart C.S., Paniagua C., Dinsdale D., Cheng K.J., Garrow S.H.: Selective isolation and characteristics of Bacteroides succinogenes from the rumen of a cow. Appl.Environ.Microbiol.41, 504–510 (1981).

    PubMed  CAS  Google Scholar 

  • Sung H.G., Kobayashi Y., Chang J.S., Ha A., Ha J.K.: Low ruminal pH reduces dietary fiber digestion via reduced microbial attachment. Asian-Austral.J.Anim.Sci.20, 200–207 (2007).

    CAS  Google Scholar 

  • Tajima K., Aminov R.I., Nagamine T., Matsui H., Nakamura M., Benno Y.: Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Appl.Environ.Microbiol.67, 2766–2774 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Weimer P.J., Waghorn G.C., Odt C.L., Mertens D.R.: Effect of diet on populations of three species of ruminal cellulolytic bacteria in lactating dairy cows. J.Dairy Sci.82, 122–134 (1999).

    Article  PubMed  CAS  Google Scholar 

  • Ziemer C.J., Sharp R., Stern M.D., Cotta M.A., Whitehead T.R., Stahl D.A.: Comparison of microbial populations in model and natural rumens using 16S ribosomal RNA-targeted probes. Appl.Environ.Microbiol.66, 632–643 (2000).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Y. Kobayashi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kobayashi, Y., Shinkai, T. & Koike, S. Ecological and physiological characterization shows that Fibrobacter succinogenes is important in rumen fiber digestion — Review. Folia Microbiol 53, 195–200 (2008).

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: