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Phytase activity and its regulation in a rhizospheric strain of Serratia plymuthica

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Abstract

Serratia plymuthica strain IC1270 isolated from the rhizosphere, possessing antagonistic activity towards a wide range of plant-pathogenic fungi, is able to hydrolyze phytate. Phytase activity was found intracellularly, while no activity was detected in the culture liquid. Optimum activity was found at pH 4–5; it completely disappeared at pH > 7.0 and 2.5. Phytase production was practically absent in the exponential phase and reached a maximum in the late stationary phase. Mutations of genes grrA and grrS, encoding GacA/GacS-like 2-component global regulatory system, or in gene rpoS encoding the σ factor RpoS subunit of RNA polymerase, led to a deficiency in phytase production. Introduction into mutants of the respective wild-type genes cloned into the wide-range plasmid pJFF224-NX under the control of the bacteriophage T4 gene 32 promoter complemented this deficiency. This is the first report implicating the GacA/GacS global regulators and RpoS factor in phytase production in bacteria.

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Abbreviations

Clm:

chloramphenicol

Gen:

gentamicin

Rif:

rifampicin

Tet:

tetracycline

PCR:

polymerase chain reaction

Clmr :

chloramphenicol-resistant

Genr :

gentamicin-resistant

Rifr :

rifampicin-resistant

Tetr :

tetracycline-resistant

PHY(s):

phytate(s)

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Authors and Affiliations

  1. Institute of Molecular Genetics, Russian Academy of Sciences, 123 182, Moscow, Russia

    E. Shedova, V. Lipasova, G. Velikodvorskaya & I. Khmel

  2. The Otto Warburg Center for Biotechnology in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100, Rehovot, Israel

    M. Ovadis & L. Chernin

Authors
  1. E. Shedova
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  2. V. Lipasova
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  3. G. Velikodvorskaya
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  4. M. Ovadis
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  5. L. Chernin
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  6. I. Khmel
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Correspondence to I. Khmel.

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Shedova, E., Lipasova, V., Velikodvorskaya, G. et al. Phytase activity and its regulation in a rhizospheric strain of Serratia plymuthica . Folia Microbiol 53, 110–114 (2008). https://doi.org/10.1007/s12223-008-0016-z

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  • DOI: https://doi.org/10.1007/s12223-008-0016-z

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