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Triangular-prism Microstructure Engineered on the Fibrous Scaffold Using Electro-centrifugal Spinning Technique for Tissue Engineering

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Abstract

Triangular-prism microstructure engineered on the surface of an aligned polycaprolactone (PCL) nanofibrous scaffold was fabricated using an electro-centrifugal spinning technique and molding approach for wound healing application. The PCL nanofiber pattern mirrored the triangular-prism microstructure of the pattern silicon substrate. The results of in vitro and in vivo experiments have indicated that the aligned nanofiber and triangular-prism in the microstructure scaffold play a synergistic role in guiding cell behaviors and responses. The peak of the triangular-prism could assist the movement of the cell and promote cell elongation behavior. By comparing with the flat scaffold in 7-day cultures, the microstructure exhibited a significant positive effect on cell behavior, including the 1.35 cell viability, 17.82±2.03 aspect ratio of cell elongation, and all cells oriented along with the triangular-prism microstructure. Moreover, this scaffold can achieve 96.81 % wound closure, and regenerated skin tissue demonstrated almost complete epithelialization. Most of the collagen fibers and proliferated fibroblast cells are arranged horizontally and mature on day 14. The results obtained from this fundamental study on the cell-surface interaction effect based on fibrous scaffolds have the potential for application in tissue engineering.

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Acknowledgements

This study was supported by a research grant from the Ministry of Science and Technology (MOST109-2221-E-110-023). The authors appreciatively acknowledge the assistance of the animal experiments from Prof. Wen, National Sun Yat-sen University, Taiwan.

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  1. Department of Mechanical and Electro-Mechanical Engineering, National Sun Yat-sen University, Kaohsiung, 804, Taiwan

    Norul Ashikin Norzain, Wei-Chih Lin & Nur Adila Mohd Razali

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  1. Norul Ashikin Norzain
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  2. Wei-Chih Lin
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Correspondence to Wei-Chih Lin.

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Norzain, N.A., Lin, WC. & Razali, N.A.M. Triangular-prism Microstructure Engineered on the Fibrous Scaffold Using Electro-centrifugal Spinning Technique for Tissue Engineering. Fibers Polym 23, 3398–3414 (2022). https://doi.org/10.1007/s12221-022-4756-1

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