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Functionalization of Electrospun Poly(Acrylonitrile-co-Styrene/Pyrrole) Copolymer Nanofibers for Using as a High-performance Carrier for Laccase Immobilization

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Abstract

Enzyme immobilization onto nanomaterials is a strategy to overcome the difficulties of the free enzymes applications where the immobilization process affords excellent reusability and operational stability against temperature and pH, and high surface area for the catalytic reaction. In this work, poly(Acrylonitrile-co-Styrene/Pyrrole), (poly(AN-co-ST/ Py)), copolymer nanofibers were successfully fabricated using the electrospinning technique. Laccase enzyme from Trametes versicolor was immobilized onto functionalized poly(AN-co-ST/Py) nanofibers by covalent attachment using polyethylenediamine (PEI) via physical adsorption and multipoint covalent attachment to the functional groups onto the polymeric surface. Since the relative activity of the immobilized Laccase depends on the amount of the coupled PEI onto the polymeric nanofibers, a colorimetric assay via copper ions complex formation was applied for the estimation of the process yield. Accordingly, the various factors affecting the PEI coupling process such as concentration, time, pH and temperature were investigated; Besides their influence on the morphology of the resultant nanofibers. Furthermore, thermal and pH stability, storage stability, and reusability were evaluated and compared. The optimum reaction temperature and pH for the immobilized enzyme were 70 °C and 6.0, respectively. The scanning electron microscopy (SEM) images showed that the modified nanofibers with PEI have kept its nanofibers structure and uniform morphology with an average diameter of approximately 559.2 nm. Within 35 days, the immobilization process reduces the activity loss from 59 % to 29 %, while thermal incubation at 50 °C for 2 hrs causes activity loss for the free and immobilized enzymes by 65 % and 30 % respectively. Therefore, the prepared copolymer nanofibers proposed in this work showed promising potential for applications to enzyme immobilization.

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Acknowledgments

This project was supported financially by the Science and Technology Development Fund (STDF), Egypt, under grant no. 15042).

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Authors and Affiliations

  1. Chemistry Department, College of Science, Jouf University, Sakaka, 2014, Saudi Arabia

    M. R. El-Aassar

  2. Polymer Materials Research Department, Advanced Technology and New Material Institute, City of Scientific Research and Technological Applications, New Borg El-Arab, 21934, Alexandria, Egypt

    M. R. El-Aassar & Ahmed A. Elzain

  3. Department of Textile Engineering, Faculty of Industries Engineering and Technology, University of Gezira, Wad Madni, P.O. Box 20, Sudan

    Mahmoud H. M. A. Shibraen

  4. Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Universities and Research Institutes District, New Borg El-Arab, 21934, Alexandria, Egypt

    Yasser R Abdel-Fattah

  5. Quality Management Sector, Central Lab, Marsa Matrouh Company for Water and Wastewater, Marsa Matrouh, Egypt

    Ahmed A. Elzain

Authors
  1. M. R. El-Aassar
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  2. Mahmoud H. M. A. Shibraen
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  3. Yasser R Abdel-Fattah
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  4. Ahmed A. Elzain
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Correspondence to M. R. El-Aassar.

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El-Aassar, M.R., Shibraen, M.H.M.A., Abdel-Fattah, Y.R. et al. Functionalization of Electrospun Poly(Acrylonitrile-co-Styrene/Pyrrole) Copolymer Nanofibers for Using as a High-performance Carrier for Laccase Immobilization. Fibers Polym 20, 2268–2279 (2019). https://doi.org/10.1007/s12221-019-9290-4

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