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Integrated Analysis of MRNA and MiRNA Expression Profiles in dys-1 Mutants of C. Elegans After Spaceflight and Simulated Microgravity

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Abstract

Dystrophin-like dys-1 gene is expressed and required in muscle tissue, playing a vital role in gravisensing in Caenorhabditis elegans (C. elegans). To date, microRNA (miRNA)-mediated epigenetic mechanism in microgravity-induced muscular atrophy remains to be elucidated. In the present study, we first analyzed mRNA and miRNA expression profiles in space-flown dys-1(cx18) mutants and wild type worms (wt) of C. elegans. The results showed that spaceflight and microgravity have fewer effects on mRNA and miRNA expression in dys-1 mutant than in wt worms. mRNA and miRNA expression patterns of dys-1 mutants were changed by microgravity. Hierarchical clustering analysis showed that the alterations of genes function on neuromuscular system under space environment. Seven miRNAs (cel-miR-52, 56, 81, 82, 84, 124 and 230) have 18 significant anti-correlated target genes under space environment. RT-qPCR analysis confirmed that miR-52 and cdh-3, miR-84 and lin-14, miR-124 and mgl-3 in dys-1 mutants reversely altered under microgravity environment and in simulated microgravity experiment. Locomotion ability was only reduced in F0 wt worms but not in dys-1 mutants as well as their F1 offspring after simulated microgravity. We observed expression alterations of 7 neuromuscular genes (unc-27, nlp-22, flp-1, egl-5, flp-4, mgl-3, unc-94) in F0 wt worms, which might be involved in the regulation of locomotion ability of C. elegans. This study provides important insights to reveal the mechanism in the pathogenesis of muscular atrophy induced by microgravity.

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The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy of this project.

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Acknowledgements

We acknowledge support from Space Science Experiment Project in Chinese Space Station (no. SCP03-01-02), and National Natural Science Foundation of China (no. 32071244).

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Authors and Affiliations

  1. College of Environment Science and Engineering, Institute of Environmental Systems Biology, Dalian Maritime University, Linghai Road 1, Dalian, 116026, P.R. China

    Jiahong Jiang, Lei Zhao, Lin Guo, Yanfang Xing, Yeqing Sun & Dan Xu

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  1. Jiahong Jiang
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  3. Lin Guo
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Contributions

Jiahong Jiang: Writing - original draft, Methodology, Investigation, Data curation, Lei Zhao, Funding acquisition, Supervision. Lin Guo: Validation, simulated microgravity experiments, prepared figures; Yanfang Xing, Validation, miRNA expression analysis, prepared tables. Yuqing Sun: Resources, Funding acquisition, Conceptualization. Dan Xu: Writing - review & editing, Project administration, Supervision. All authors reviewed the manuscript.

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Correspondence to Dan Xu.

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Jiang, J., Zhao, L., Guo, L. et al. Integrated Analysis of MRNA and MiRNA Expression Profiles in dys-1 Mutants of C. Elegans After Spaceflight and Simulated Microgravity. Microgravity Sci. Technol. 35, 31 (2023). https://doi.org/10.1007/s12217-023-10057-w

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