Abstract
The cardiovascular system is assumed to be one mostly affected by space flight. Endothelial cells (ECs) are involved significantly into adaptation of the vascular tree to negative effects of space flight, microgravity in particular. The basal level of EC activity could determine the response of EC lining to real or simulated microgravity. Here we examined the effects of short-term 24 h exposure at random positioning machine (RPM) in order to simulate microgravity (sµg) on inflammatory activation by TNF-α and the combined effect thereof on interleukin transcription/production in ECs. EC samples were discriminated into groups relating to high (ECs-1) or low (ECs-2) basal ratio of ICAM-1 positive cells. ECs-1 displayed a higher basal level of IL-8, while the elevation after TNF-α exposure was more pronounced in ECs-2 (3.3 vs 15 folds). TNF-α-induced IL-6 elevation was 2.9- and 7.1-folds in ECs-1 and ECs-2 respectively. A short-term sµg had no effect on IL-6 and IL-8 production and on IL6 and IL8 transcription in ECs of both groups. Combined sµg-TNF-α exposure did not affect TNF-induced elevation of interleukin levels. Herewith, more pronounced upregulation of IL8 and downregulation of IL6 in ECs-2 was observed. Above data suggest that ECs-1 are in a pre-activated and/or increased reactivity state, while ECs-2 are capable to react on microenvironmental alterations more properly. Thereby, the use of primary cultures of endothelial cells for space flight or simulated microgravity experiments requires a more precise evaluation of their functional steady state.
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Acknowledgements
This work was supported in part by the Basic Research Program of the Institute of Biomedical Problems Russian Academy of Sciences (project no. 65.3) and by the Russian Science Foundation (project no. 16-15-10407).
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This work was supported in part by Program of Basic Research of IMBP RAS (project no. 65.3) and by the Russian Science Foundation (project no. 16–15-10407).
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Evgeny G. Rudimov and Yulia V. Rudimova performed the experiments and analyzed the data; Evgeny G. Rudimov and Yulia V. Rudimova, Elena R. Andreeva wrote original manuscript; Lyudmila B. Buravkova supervised the design and execution of the study and critically revised the manuscript.
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Rudimov, E.G., Rudimova, Y.V., Andreeva, E.R. et al. Simulated Microgravity Affects the TNF-α-Induced Interleukin Profile of Endothelial Cells Depending on the Initial ICAM-1 Expression. Microgravity Sci. Technol. 34, 1 (2022). https://doi.org/10.1007/s12217-021-09920-5
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DOI: https://doi.org/10.1007/s12217-021-09920-5