Abstract
The accumulation of plant pigments in mung bean (Vigna radiata L.) seedlings was measured after clinorotation (2 rpm for 2-4 days), and compared to a stationary control. The pigments measured included chlorophyll and carotenoid in primary leaves, and the anthocyanin in seedlings. While significant changes in chlorophyll and carotenoid accumulation were not observed during the initial 2 to 4 days of cultivation, by day 4 the seedlings grown on the clinostat had lower levels of anthocyanin, compared to those in the control seedlings. To further detail the cause for the observed reduction in anthocyanin accumulation under altered gravity conditions, seedlings were grown in the presence of silver nitrate, a known ethylene inhibitor, for 4 days, since it is known ethylene has a negative impact on anthocyanin accumulation. Silver nitrate promoted anthocyanin accumulation in the clinostat seedlings, and as a result there was no significant difference between the control and clinostat seedlings in anthocyanin accumulation. The results suggest that slow clinorotation negatively impacts anthocyanin pigmentation in mung bean seedlings, with endogenous ethylene suspected to be involved in this.
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Abbreviations
- Chl:
-
Chlorophyll
- CL:
-
Clinostat
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Acknowledgements
The authors thank Prof. Kensuke Miyamoto (Osaka Prefecture University) and Prof. Junichi Ueda (Osaka Prefecture University) for providing important suggestions and discussion; Prof. Hiroshi Shimizu (Kyoto University) for using him photo meter; Prof. Tateshi Fujiura (Kyoto University) for providing AC servo motors; Prof. Garry John Piller (Kyoto University) for reading this report and helpful suggestions. This work was supported by JSPS KAKENHI grant number 16H05010.
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Nakajima, S., Shiraga, K., Suzuki, T. et al. Chlorophyll, Carotenoid and Anthocyanin Accumulation in Mung Bean Seedling Under Clinorotation. Microgravity Sci. Technol. 29, 427–432 (2017). https://doi.org/10.1007/s12217-017-9561-x
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DOI: https://doi.org/10.1007/s12217-017-9561-x