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Response of Haloalkaliphilic Archaeon Natronococcus Jeotgali RR17 to Hypergravity

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Abstract

The survival of archaeabacteria in extreme inhabitable environments on earth that challenge organismic survival is ubiquitously known. However, the studies related to the effect of hypergravity on the growth and proliferation of archaea are unprecedented. The survival of organisms in hypergravity and rocks in addition to resistance to cosmic radiations, pressure and other extremities is imperative to study the possibilities of microbial travel between planets and endurance in hyperaccelerative forces faced during ejection of rocks from planets. The current investigation highlights the growth of an extremophilic archaeon isolated from a rocky substrate in hypergravity environment. The haloalkaliphilic archaeon, Natronococcus jeotgali RR17 was isolated from an Indian laterite rock, submerged in the Arabian sea lining Coastal Maharashtra, India. The endolithic haloarchaeon was subjected to hypergravity from 56 – 893 X gusing acceleration generated by centrifugal rotation. The cells of N. jeotgali RR17 proliferated and demonstrated good growth in hypergravity (223 X g). This is the first report on isolation of endolithic haloarchaeon N. jeotgali RR17 from an Indian laterite rock and its ability to proliferate in hypergravity. The present study demonstrates the ability of microbial life to survive and proliferate in hypergravity. Thus the inability of organismic growth in hypergravity may no longer be a limitation for astrobiology studies related to habitability of substellar objects, brown dwarfs and other planetary bodies in the universe besides planet earth.

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Acknowledgments

This research was supported by a grant from Indian Space Research Organization (ISRO) –Space Technology Cell-University of Pune (STC-UoP), Grant no. 140 to RT. VS thanks ISRO for Junior research fellowship. We thank Dr. P.P. Kanekar for suggestions regarding sampling site and sampling and Ms. Oke R for biochemical characterization. We thank Principal, Modern College, Shivajinagar, Pune for providing necessary facilities for the work and Prof. Dr. P.D. Sabale, Dept. of Archaeology, Deccan College, Pune for help in identification of rock.

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  1. Department of Biotechnology, Modern College of Arts, Science and Commerce, Shivajinagar, Pune, 411005, Maharashtra, India

    Rebecca S. Thombre, Aniruddha R. Bhalerao & Vinaya D. Shinde

  2. Microbial Culture Collection (MCC), National Centre for Cell Science, Ganeshkhind, Pune, 411007, India

    Sunil Kumar Dhar & Yogesh S. Shouche

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  1. Rebecca S. Thombre
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Correspondence to Rebecca S. Thombre.

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Thombre, R.S., Bhalerao, A.R., Shinde, V.D. et al. Response of Haloalkaliphilic Archaeon Natronococcus Jeotgali RR17 to Hypergravity. Microgravity Sci. Technol. 29, 191–200 (2017). https://doi.org/10.1007/s12217-017-9538-9

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