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Small RNA Deep Sequencing and the Effects of microRNA408 on Root Gravitropic Bending in Arabidopsis

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Abstract

MicroRNA (miRNA) is a non-coding small RNA composed of 20 to 24 nucleotides that influences plant root development. This study analyzed the miRNA expression in Arabidopsis root tip cells using Illumina sequencing and real-time PCR before (sample 0) and 15 min after (sample 15) a 3-D clinostat rotational treatment was administered. After stimulation was performed, the expression levels of seven miRNA genes, including Arabidopsis miR160, miR161, miR394, miR402, miR403, miR408, and miR823, were significantly upregulated. Illumina sequencing results also revealed two novel miRNAsthat have not been previously reported, The target genes of these miRNAs included pentatricopeptide repeat-containing protein and diadenosine tetraphosphate hydrolase. An overexpression vector of Arabidopsis miR408 was constructed and transferred to Arabidopsis plant. The roots of plants over expressing miR408 exhibited a slower reorientation upon gravistimulation in comparison with those of wild-type. This result indicate that miR408 could play a role in root gravitropic response.

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Acknowledgments

This work was supported by the National Key Basic Research and Development Program (2011CB710902). Center for the Application of Molecular Biology of International Agriculture, Australia is gratefully acknowledged for supplying the expression vector pCAMBIA-2300 vector.

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Authors and Affiliations

  1. Molecular Biology Laboratory, China Academy of Space Technology, Beijing, China

    Huasheng Li, Jinying Lu, Qiao Sun, Yu Chen & Min Liu

  2. Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, China

    Min Liu

  3. School of Life Sciences, Beijing Normal University, Beijing, China

    Dacheng He

Authors
  1. Huasheng Li
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  2. Jinying Lu
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  3. Qiao Sun
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  4. Yu Chen
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  5. Dacheng He
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  6. Min Liu
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Corresponding author

Correspondence to Min Liu.

Additional information

The first two authors (Jinying Lu and Huasheng Li,) contribute equally to this work.

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Li, H., Lu, J., Sun, Q. et al. Small RNA Deep Sequencing and the Effects of microRNA408 on Root Gravitropic Bending in Arabidopsis . Microgravity Sci. Technol. 27, 495–503 (2015). https://doi.org/10.1007/s12217-015-9444-y

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  • DOI: https://doi.org/10.1007/s12217-015-9444-y

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