Abstract
We describe a custom-made two electrode voltage clamp (TEVC) system suitable for real-time recording from Xenopus laevis oocytes in microgravity research applications. This miniaturized version of a standard laboratory TEVC, has proven to be reliable and robust. It allows the recording of membrane currents of comparable quality to ground-based TEVC measurements. The data presented were obtained during an ESA parabolic flight campaign (Bordeaux, France) and suggests that differing gravity conditions can reversibly modulate transmembrane ionic current in Xenopus laevis oocytes.
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Richard, S., Henggeler, D., Ille, F. et al. A Semi-automated Electrophysiology System for Recording from Xenopus Oocytes Under Microgravity Conditions. Microgravity Sci. Technol. 24, 237–244 (2012). https://doi.org/10.1007/s12217-012-9307-8
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DOI: https://doi.org/10.1007/s12217-012-9307-8