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PMT (Photomultiplier) Clinostat

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In order to enable kinetic online measurements in cell cultures during exposure to altered gravitational stimulation a new device has been constructed. The analysis principle is based on photon counting with a photomultiplier tube (PMT). The system can work in a rotating mode (clinostat principle) as well as in 1 g conditions. Biological verification was successfully performed with a Luminol-based luminescence measurement of the immune reaction of mammal macrophages. An identical methodical approach is foreseen for an ISS experiment (TRIPLE LUX). Alteration of the rotation speed of the PMT clinostat (60 and 2 rotations per minute—rpm) resulted in a speed-dependent decrease of the luminescent signal, contributing to the current discussion whether and how fast rotation of a sample around one axis perpendicular to the direction of the g-vector provides the condition of functional weightlessness or omnilateral mechano-(gravi-) stimulation.

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  • Allen, R.C.: Phagocytic leukocyte oxygenation activities and chemiluminescence: a kinetic approach to analysis. Methods Enzymol. 133, 449–493 (1986)

    Article  Google Scholar 

  • Briegleb, W.: Ground-borne methods and results in gravitational cell biology. Physiologist 31, 44–47 (1988)

    Google Scholar 

  • Briegleb, W.: Some qualitative and quantitative aspects of the fast-rotating clinostat as a research tool. ASGSB Bulletin 5(2), 23–30 (1992)

    Google Scholar 

  • Hensel, W., Sievers, A.: Effects of prolonged omnilateral gravistimulation on the ultrastructure of statocytes and on the graviresponse of roots. Planta 150, 338–346 (1980)

    Article  Google Scholar 

  • Huber, K.: Phagocytose und oxidativer Burst als Biomarker für Immunotoxizität, Dissertation, Lehrstuhl für Zellbiologie der Technischen Universität München, Wissenschaftszentrum Weihenstephan (2007)

  • Klaus, D.M., Todd, P., Schatz, A.: Functional weightlessness during clinorotation of cell suspensions. Adv. Space Res. 21, 1315–1318 (1998)

    Article  Google Scholar 

  • Ullrich, O., Huber, K., Lang, K.: Signal transduction in cells of the immune system in microgravity. Cell Commun. Signal. 6, 9 (2008)

    Article  Google Scholar 

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Correspondence to Astrid Horn.

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Horn, A., Ullrich, O., Huber, K. et al. PMT (Photomultiplier) Clinostat. Microgravity Sci. Technol. 23, 67–71 (2011).

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