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Rendiconti Lincei. Scienze Fisiche e Naturali

, Volume 29, Issue 3, pp 675–690 | Cite as

Distinctive antagonistic role of new Enterococcus faecium ER-3M strain and its bacteriocin effect against Staphylococcus aureus Pneumonia

  • Marwa S. Abdel-HamidEmail author
  • Anis Anis
  • Rania H. Elbawab
  • Abeer A. B. Mohammed
  • Sahar H. Orabi
  • Said I. Fathalla
Article
  • 156 Downloads

Abstract

Bacteriocins secreted by some bacteria such as Enterococcaceae has a protected role against Staph. aureus pneumonia. This study identified a new Enterococcus sp. that produces efficient bacteriocin which attenuates Staph. aureus pneumonia in rats. Identification of Enterococcus sp. was achieved according to Bergey’s manual of systematic bacteriology using 16S rRNA analysis. Bacteriocin was extracted, partially purified and characterized. Antagonistic activity of the bacterial cells or its bacteriocin was estimated in vitro against different pathogenic bacteria and in vivo with an animal experiment, where histopathological and hematological examinations were evaluated. The isolate was identified as Ent. faecium ER-3M, with accession number KY774316. It exhibits broad antimicrobial spectrum against Gram-positive and Gram-negative organisms in vitro. Treatment of rats with bacterial cells or bacteriocin (500 Au mg−1), previously infected with Staph. aureus, significantly decreased the symptoms, WBCs, neutrophils, and liver functions. Histopathological examination of rat lung tissues revealed that treatment with probiotic bacteria and/or its bacteriocin can attenuate the pneumonic lesions caused by Staph. aureus bacteria. Partially purified bacteriocin was characterized by a large molecular weight of approximately 40 kDa a proteinous nature according to both Fourier transform infrared spectrometry (FTIR) spectroscopy and 1H-Nuclear magnetic resonance (NMR) analysis and belongs to class III of bacteriocins termed enterolysin. Based on the results, the successful treatment of Ent. faecium ER-3M or its bacteriocin may be one of the promising treatments against Staph. aureus pneumonia.

Keywords

Enterococcus faecium Bacteriocin Antagonistic activity Staphylococcus aureus pneumonia 

Notes

Acknowledgements

Special thanks go to Dr. Ahmed M. Hammad Associative professor, Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Sadat City, Egypt, whose comments was very helpful during bacterial isolation stage though this study.

Compliance with ethical standards

Conflict of interest

The authors declared that the present study was performed in absence of any conflict of interest.

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Copyright information

© Accademia Nazionale dei Lincei 2018

Authors and Affiliations

  1. 1.Microbial Biotechnology DepartmentUniversity of Sadat City, Genetic Engineering and Biotechnology Research InstituteSadat CityEgypt
  2. 2.Pathology Department, Faculty of Veterinary MedicineUniversity of Sadat CitySadat CityEgypt
  3. 3.Ministry of Health, Health Insurance Organization (HIO)Sadat City ClinicSadat CityEgypt
  4. 4.Biochemistry and Chemistry of Nutrition Department, Faculty of Veterinary MedicineUniversity of Sadat CitySadat CityEgypt
  5. 5.Physiology Department, Faculty of Veterinary MedicineUniversity of Sadat CitySadat CityEgypt

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