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Efficient protocol for in vitro propagation from bulb scale explants of Fritillaria ruthenica Wikstr. (Liliaceae), a rare ornamental species

  • Dinara S. Muraseva
  • Tatyana I. Novikova
Article

Abstract

An efficient protocol for in vitro propagation from bulb scale explants has been developed for Fritillaria ruthenica Wikstr., an endangered and rare species. The use of B5 medium supplemented with 5.0-µM 6-benzylaminopurine (BAP) and 2.0-µM α-naphthalene acetic acid (NAA) was found to be the most effective for adventitious bud induction and shoot multiplication (2.9 shoots per explant with 100% regeneration frequency). Adventitious regeneration occurred exclusively via direct organogenesis with the development of microshoots consisting of the rosette leaves, while bulb formation was not observed. The rooting microplants was carried out on a half-strength BDS hormone-free medium supplemented with activated charcoal at a concentration of 0.5 g/l resulted in 100% rhizogenesis with 5.2 roots per plantlet. Elevated sucrose concentrations (40.0 and 50.0 g/l) had no stimulating effect on bulblet differentiation at the in vitro rooting stage of F. ruthenica shoots. The regenerated plantlets were successfully transferred in a mixture of coconut fiber and sand (3:1) for acclimatization in greenhouse conditions with 72% of survival rate. The large bulbs developed only in ex vitro conditions at the end of the vegetation period.

Keywords

In vitro propagation Fritillaria ruthenica Rare species Scale explants Bulblet development 

Notes

Acknowledgements

In our study, material from the collection of the Central Siberian Botanical Garden SB RAS—USU_440534 “Collection of living plants indoors and outdoors” was used.

Funding

The work was carried out with the financial support of the budgetary project of the Central Siberian Botanical Garden, SB RAS “Assessment of morphogenetic potential of North Asian plant populations by experimental methods” (no. AAAA-A17-117012610051-5) within the framework of the State Assignment.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Accademia Nazionale dei Lincei 2018

Authors and Affiliations

  1. 1.Central Siberian Botanical Garden, Siberian Branch RASNovosibirskRussia

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