Evaluation of in vitro antioxidant activity, HPLC and GC–MS analysis along with chemoprofiling of Decalepis arayalpathra: a critically endangered plant of Western Ghats, India
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Decalepis arayalpathra popularly used as an effective remedy for peptic ulcer, gastric antisecretory and cancer-like afflictions with an immunomodulatory property. This study was conducted to unravel the chemical composition of the tuberous root and bioactivities of the compounds in the fractions of antioxidant activity by using large-scale multi-targeted approach, which is still unexplored. Methanolic extract (EM) and its sequential fraction (EC: chloroform extract, EEA: ethyl acetate extract, EB: butanol extract, EA: aqueous extract) were used for qualitative and quantitative evaluation of phytocompounds present in the tuberous root of the plant. The characterization of 2H4MB and chlorogenic acid by using high-performance liquid chromatography (HPLC) was also performed with the help of standard compound. The results revealed the extract to be rich in phenolics, tannins, flavonoids, carbohydrate, saponin and alkaloids. EM proved to be a compatible solvent for the extraction and corresponds to maximum numbers of phytocompounds followed by its fraction (EB, EA, EEA and EC). EM, EB and EEA were further subjected for gas chromatography–mass spectrometry (GC–MS) analysis, which revealed the presence of more than 20 different bioactive compounds. The methanolic extract and its successive fractions were also screened for the antioxidant activity using different prototype. 2H4MB and chlorogenic acid quantified by HPLC in methanol extract was 11.22 and 9.67 μg/ml respectively. The reported compound might be responsible for the potent antioxidant property of the plant. To our knowledge, this is the first report on the analysis of the chemical composition and the bioactive potential of the compounds obtained by extract of D. arayalpathra.
Keywords2H4MB Antioxidant activity Bioactivities GC–MS HPLC
Financial assistance in the form of junior research fellow under DST-PURSE program Vide No. F.6-5-EP/DS/224) to Zishan Ahmad is gratefully acknowledged. Dr. Shiwali Sharma gratefully acknowledges the financial assistance provided by the Department of Science and Technology (DST) in the form of Young Scientist Project under the Fast Track Scheme, SERB (Vide No. SB/FT/LS-364/2012).
This work was carried out in collaboration among three authors. AS conceived and designed all the experiments. SS helped for the quantitative estimation of the chemical compound. ZA performed all the experimental work, analyzed the data and wrote the manuscript. All the authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
There is no conflict of interest among the authors.
- Anonymous (2011) Tamil Nadu State Biodiversity Notification, Ministry of Environment and Forests, New Delhi, India. REGD. No. D.L- 33004/99Google Scholar
- CAMP-1 (1995) The first red list of medicinal plants of South India, Foundation for Revitalization of Local Health Tradition (FRLHT). Bangalore, IndiaGoogle Scholar
- Davidson PM, Naidu AS (2000) Phyto-phenols. In: Naidu AS (ed) Natural food antimicrobial systems. CRC Press LLC, Boca Raton, pp 265–294Google Scholar
- Hagerman A, Harvey-Muller I, Makkar HPS (2000) Quantification of tannins in tree foliageda laboratory manual, vol 4. FAO/IAEA, Vienna, p 7Google Scholar
- Joseph J, Chandrasekaran V (1978a) Janakia arayalpathra- a new genus and species of Periplocaceae from Kerala, South India. J Ind Bot Soci 57:308–312Google Scholar
- Joseph J, Chandrasekhran V (1978b) A new species of Periplocaceae from Kerala, South India. Bot Soc 57: 308–312(London 12–16)Google Scholar
- Khandelwal KR (2007) Practical pharmaconosy. Techniques and experiments. Pune, India: Nirali Prakashan 149Google Scholar
- Pushpangadan P, Rajasekaran S, Ratheesh-Kumar PK, Jawahar CR, Radhakrishnan K, Nair CPR, Sarada-Amma L, Bhat AV (1990) Amrithapala (Janakia arayalpathra, Joseph and Chandrasekharan), a new drug from the Kani Tribe of Kerala. Ancient Sci Life 9:215–219Google Scholar
- Son KH, Kwon SY, Kim HP, Chang HW, Kang SS (1998) Constituents from Syzygium aromaticum Merr Et Perry. Nat Prod Sci 44:263–267Google Scholar
- Subramoniam A, Rajasekharan S, Latha PG, Evans DA, Pushpangadan P (1998) Immunomodulatory and antitumour activities of Janakia arayalpathra root. Fitoterapia 68:140–144Google Scholar