Does synthetic seed storage at higher temperature reduce reserpine content of Rauvolfia serpentina (L.) Benth. ex Kurz.?
- 70 Downloads
In the present study, we analyzed the impact of storage temperature and storage duration of synthetic seeds of Rauvolfia serpentina (L.) Benth. ex Kurz. on their germination performance and reserpine content, its key secondary metabolite. Synthetic seeds were developed using in vitro shoot tips (3–4 mm long) polymerized with 3% (w/v) sodium alginate and 75 mM calcium chloride. Storage of synthetic seeds at 25 °C exhibited a high frequency of germination (82%) after 30-day storage. However, longer storage period (60 days) drastically reduced the germination frequency of synthetic seeds to 36%. Alternatively, in case of 8 °C storage temperature, 60% synthetic seeds were germinated after 30-day storage with a minor decline to 52% following 60-day storage. Moreover, synthetic seed-germinated plantlets from 25 °C storage condition showed a regression in reserpine content (203.38 ± 0.21 µg gm−1 of dried sample) than that of the plantlets regenerated from 8 °C storage (249.37 ± 0.21 µg gm−1 of dried sample). Our study clearly infers that a lower temperature condition (above freezing; 8 °C) is appropriate for storage, post-storage germination, and upholding the reserpine content of R. serpentina synthetic seeds.
KeywordsCalcium chloride Encapsulation Germination frequency Germplasm exchange Reserpine Sodium alginate
High-performance thin-layer chromatography
Murashige and Skoog (1962)
The authors are thankful to the Plant Tissue Culture Laboratory, Faculty Centre for Integrated Rural Development and Management (IRDM), School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda University (RKMVU). The authors are grateful to Dr. Alok Kumar Hazra and Mr. Soumya Mandal (RKM Quality Testing Laboratory, Faculty Centre for IRDM, RKMVU) for their assistance during phytochemical assessment.
SG: conceived the idea and designed the experiments; SG and SK: executed all the experiments and statistical analysis; SK: wrote the initial draft of the manuscript; SG: scrutinized and corrected the manuscript to its final version. Both the authors read and approved the manuscript prior to its submission.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Compton ME (1994) Statistical methods suitable for the analysis of plant tissue culture data. Plant Cell Tissue Org Cult 37:217–242Google Scholar
- Danso KE, Ford-Lloyd BV (2003) Encapsulation of nodal cuttings and shoot tips for storage and exchange of cassava germplasm. Plant Cell Rep 21:718–725Google Scholar
- Gantait S, Kundu S, Yeasmin L, Ali N (2017a) Impact of differential levels of sodium alginate, calcium chloride and basal media on germination frequency of genetically true artificial seeds of Rauvolfia serpentina (L.) Benth. ex Kurz. J Appl Res Med Aromat Plants 4:75–81Google Scholar
- Ghani A (1998) Medicinal plants of Bangladesh. Chemical constituents and uses, 2nd edn. Asiatic Soc, Bangladesh, p 36Google Scholar
- Ikhlaq M, Hafiz IA, Micheli M, Ahmad T, Abbasi NA, Standardi A (2010) In vitro storage of synthetic seeds: effect of different storage conditions and intervals on their conversion ability. Afr J Biotechnol 9:5712–5721Google Scholar
- Stanford JL, Martin EJ, Brinton LA, Hoover RN (1986) Rauwolfia use and breast cancer: a case–control study. J Natl Cancer Inst 76:817–822Google Scholar
- The Wealth of India (1950) A dictionary of indian raw materials and industrial products. Raw materials Vol. VIII, Publication and Information Directorate, CSIR, New Delhi, pp. 376–391Google Scholar