Rendiconti Lincei

, Volume 28, Issue 4, pp 679–686 | Cite as

Does synthetic seed storage at higher temperature reduce reserpine content of Rauvolfia serpentina (L.) Benth. ex Kurz.?



In the present study, we analyzed the impact of storage temperature and storage duration of synthetic seeds of Rauvolfia serpentina (L.) Benth. ex Kurz. on their germination performance and reserpine content, its key secondary metabolite. Synthetic seeds were developed using in vitro shoot tips (3–4 mm long) polymerized with 3% (w/v) sodium alginate and 75 mM calcium chloride. Storage of synthetic seeds at 25 °C exhibited a high frequency of germination (82%) after 30-day storage. However, longer storage period (60 days) drastically reduced the germination frequency of synthetic seeds to 36%. Alternatively, in case of 8 °C storage temperature, 60% synthetic seeds were germinated after 30-day storage with a minor decline to 52% following 60-day storage. Moreover, synthetic seed-germinated plantlets from 25 °C storage condition showed a regression in reserpine content (203.38 ± 0.21 µg gm−1 of dried sample) than that of the plantlets regenerated from 8 °C storage (249.37 ± 0.21 µg gm−1 of dried sample). Our study clearly infers that a lower temperature condition (above freezing; 8 °C) is appropriate for storage, post-storage germination, and upholding the reserpine content of R. serpentina synthetic seeds.


Calcium chloride Encapsulation Germination frequency Germplasm exchange Reserpine Sodium alginate 



High-performance thin-layer chromatography


Murashige and Skoog (1962)



The authors are thankful to the Plant Tissue Culture Laboratory, Faculty Centre for Integrated Rural Development and Management (IRDM), School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda University (RKMVU). The authors are grateful to Dr. Alok Kumar Hazra and Mr. Soumya Mandal (RKM Quality Testing Laboratory, Faculty Centre for IRDM, RKMVU) for their assistance during phytochemical assessment.

Author contributions

SG: conceived the idea and designed the experiments; SG and SK: executed all the experiments and statistical analysis; SK: wrote the initial draft of the manuscript; SG: scrutinized and corrected the manuscript to its final version. Both the authors read and approved the manuscript prior to its submission.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12210_2017_637_MOESM1_ESM.xls (37 kb)
Supplementary material 1 (XLS 37 kb)


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Copyright information

© Accademia Nazionale dei Lincei 2017

Authors and Affiliations

  1. 1.All India Coordinated Research Project on Groundnut, Directorate of ResearchBidhan Chandra Krishi ViswavidyalayaNadiaIndia
  2. 2.Department of Genetics and Plant Breeding, Faculty of AgricultureBidhan Chandra Krishi ViswavidyalayaNadiaIndia
  3. 3.Department of Agricultural Biotechnology, Faculty Centre for Integrated Rural Development and Management, School of Agriculture and Rural DevelopmentRamakrishna Mission Vivekananda UniversityNarendrapurIndia
  4. 4.Department of Agricultural Biotechnology, Faculty of AgricultureBidhan Chandra Krishi ViswavidyalayaNadiaIndia

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