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Structural Characterization of Glucan Produced by Lactobacillus sake L-7 from Sausage

  • Fang Feng
  • Qingqing Zhou
  • Yanfang Yang
  • Fangkun Zhao
  • Renpeng Du
  • Ye Han
  • Zhijiang Zhou
Research Article
  • 41 Downloads

Abstract

A linear glucan was produced by Lactobacillus sake L-7 isolated from homemade sausage. Cultivation of the strain in Man–Rogosa–Sharpe (MRS) medium containing 50 g/L sucrose yielded 5.3 g/L of purified exopolysaccharide (EPS). The EPS was characterized by gas chromatography (GC), Fourier-transform infrared (FT-IR) spectroscopy, high-performance size-exclusion chromatography (HPSEC), nuclear magnetic resonance (NMR) spectroscopy, and scanning electron microscopy (SEM). The monosaccharide composition of the EPS was glucose, and its molecular weight was 1 × 107 Da. The FT-IR and NMR spectra revealed that the L-7 EPS was a linear glucan with α-(1 → 6) glucosidic bonds. SEM images of the dried EPS revealed a hollow tubular structure. The water solubility index and water holding capacity of L-7 EPS were 96 and 272%, respectively. The results of hydrolysis indicated that L-7 EPS was not susceptible to hydrolysis by physiological barriers and can be used as a soluble dietary fiber with health benefits. All these characteristics suggest that L-7 EPS might have potential applications in the food, cosmetic, and pharmaceutical industries.

Keywords

Glucan Exopolysaccharide Structural characterization Lactobacillus sake Hydrolysis 

Notes

Acknowledgements

The work was supported by National Science and Technology Support Program of China (No. 2015BAD16B01).

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Copyright information

© Tianjin University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Fang Feng
    • 1
  • Qingqing Zhou
    • 1
  • Yanfang Yang
    • 1
  • Fangkun Zhao
    • 1
  • Renpeng Du
    • 1
  • Ye Han
    • 1
  • Zhijiang Zhou
    • 1
  1. 1.School of Chemical Engineering and TechnologyTianjin UniversityTianjinChina

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