Transactions of Tianjin University

, Volume 23, Issue 3, pp 211–220 | Cite as

Fecal Microbiota Succession of Piglets from Birth to Post-weaning by 454 Pyrosequencing Analysis

Research article


The objective of this study was to describe the fecal microbiota succession of piglets from birth to post-weaning by pyrosequencing. The dominant bacterial phyla were Bacteroidetes, Firmicutes, Proteobacteria, and Fusobacteria, although the composition of the fecal microbiota changed as the piglets grew. Bacteroides, Parabacteroides, Prevotella, Lactobacillus, unclassified Lachnospiraceae, Ruminococcus, Oscillospira, Phascolarctobacterium, and Desulfovibrio were the core genera in the feces of piglets and existed from birth to post-weaning. Bacteroides was the most abundant at birth; however, the proportion of Bacteroides decreased as the piglets aged, while the proportion of Prevotella increased until it became the most abundant genus at the post-weaning stage. Principal coordinate analysis indicated that the microbial communities of piglet fecal samples from birth to post-weaning could be separated into four groups according to the similarity of community composition. These corresponded to time points on day 0 (birth); day 7; days 14, 21, and 28 (pre-weaning); and day 35 (post-weaning). This study suggests that the development of the intestinal microbiota in the piglet is a gradual and sequential process. This methodology establishes a basis for broader studies to investigate the microbiota of the piglet intestinal tract.


Succession Fecal microbiota Piglet Pyrosequencing Weaning 



This work was supported by the Open Funding Project of the Key Laboratory of Systems Bioengineering in Tianjin, Ministry of Education of China (No. 20160110).


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Copyright information

© Tianjin University and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.School of Chemical Engineering and TechnologyTianjin UniversityTianjinChina
  2. 2.Tianjin Institute of Animal Husbandry and Veterinary ResearchTianjinChina
  3. 3.Tianjin Kuntai Environment & Energy Science and Technology Development Co.,LtdTianjinChina
  4. 4.Key Laboratory of Systems Bioengineering, Ministry of EducationTianjin UniversityTianjinChina

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