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Nano-magnetothermal effect enhances the glucose oxidase activity of FVIOs-GOD in antibacterial research

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Abstract

In this study, glucose oxidase (GOD) was fixed on ferrimagnetic vortex-domain iron oxide nanorings (FVIOs) using the covalent bond crosslinking method to create FVIOs-GOD compounds. The activity of surface-conjugated enzymes can be effectively and selectively enhanced by utilizing FVIOs to induce magnetothermal effects under the influence of alternating magnetic fields. GOD can further catalyze the oxidation of glucose to hydrogen peroxide (H2O2), which possesses bactericidal properties. This study presents a comprehensive technique for controlling the activity of enzymes by nano-magnetothermal stimulation, which was utilized in Escherichia coli antibacterial studies. In comparison to the control group, the enzyme activity of the FVIOs-GOD immobilized enzymes rose by 50 % due to the nano-magnetothermal stimulation, and the enzyme activity could be controlled by varying the magnetic field intensity. The antibacterial experiments indicated that the survival rate of Escherichia coli decreased by 55 % after exposure to a magnetic field.

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Acknowledgments

This work was supported by grants from The National Natural Science Foundation of China (92048202), Innovation Capacity Support Plan of Shaanxi Province (No. 2020TD-040) and Free Exploration and Innovation Program of the First Affiliated Hospital of Xi’an Jiaotong University (2021ZYTS-11).

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Authors and Affiliations

  1. Institute of Regenerative and Reconstructive Medicine, Med-X Institute, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710049, China

    Qianqian Tang, Nana Zhang, Nan Zhang, Xiaoli Liu, Yi Lv & Rongqian Wu

  2. National Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, China

    Qianqian Tang, Nana Zhang, Nan Zhang, Xiaoli Liu, Yi Lv & Rongqian Wu

  3. College of Chemistry and Materials Science, Northwest University, Xi’an, 710127, China

    Ran Xiong

  4. Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, China

    Yi Lv

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  1. Qianqian Tang
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  2. Ran Xiong
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  3. Nana Zhang
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  4. Nan Zhang
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  7. Rongqian Wu
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Correspondence to Rongqian Wu.

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Qianqian Tang is an intern researcher of Institute of Regenerative and Reconstructive Medicine, First Affiliated Hospital of Xi’an Jiaotong University. She received her master’s degree from Northwestern University. Her research interests include the regulation of biomacromolecules by the magnetothermal effect of nanoparticles and immunotherapy.

Rongqian Wu is the Deputy Director of the Institute of Advanced Surgical Technology and Engineering of Xi’an Jiaotong University and one of the first batch of young top talents of Xi’an Jiaotong University. In 1996, he received his master’s degree from Xi’an Medical University. In 1999, he graduated from the Military Medical Training School of PLA General Hospital with a Doctor’s degree in general surgery. His research interests include surgical critical illness and combination therapy.

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Tang, Q., Xiong, R., Zhang, N. et al. Nano-magnetothermal effect enhances the glucose oxidase activity of FVIOs-GOD in antibacterial research. J Mech Sci Technol 38, 1601–1611 (2024). https://doi.org/10.1007/s12206-024-0250-6

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